background: typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. aim of current study was to evaluate a shortened time –multiplex pcr for rapid detection of different sal monella enterica serovars. methods: the pcr primers for three target genes tyv, prt and inva were subjected for amplification by pcr. by using sim ple dna ...

background: multidrug resistance in salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. methods: salmonella strains isolated from poultry samples by biochemical positive and negative tests were subjected to pcr and identified as salmonella enteritidis. for detection and identi...

this survey of salmonella and specifically salmonella enteritidis contamination in broiler flocks carried out in amol as one of the most important poultry breeding centers, used as manure, yolk sac, papers and visceral organs from dead birds. standard cultural methods were used for salmonella isolation. slide agglutination or tube agglutination tests were erformed using salmonella somatic o pol...

Background and aims: Salmonella spp. infect cold-blooded and warm-blooded animals and maycause a worldwide zoonotic disease, salmonellosis, in infected animals. Rodents can be Salmonellacarriers without any signs of salmonellosis. The frequency of salmonella contamination, the presence ofvirulence genes (SpvR and SpvB) and antibiotic resistance pattern of isolated Salmonella w...

Poultry meat has been identified as one of the principal foodborne sources of Salmonella. In this preliminary study the prevalence of Salmonella spp. and its typhimurium serovar contamination of broiler carcasses, were determined. Using the rinse test method, numbers of 60 samples, representing 20 broiler flocks, were collected from poultry carcasses after the chilling stage in the processing l...

The present study was designed to investigate the prevalence of Salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. A total of 1125 samples were collected from backyard chickens in two consequent samplings. In the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

plasmids play an important role in the virulence and antibiotic resistance of salmonella. salmonella carry plasmids having different size and number. plasmid profile analysis of single isolates of salmonella typhimurium and salmonella enteritidis revealed the presence of 4- plasmid profiles. salmonella serovars produce several type iii secretions including sop-e and sop –b. soap e is detected b...