Streptococcus iniae is a beta-hemolytic, Gram-positive coccus, which affects a broad range of freshwater and marine fish species, causing substantial economic losses in the aquaculture industry worldwide. Thus, it is very important to derive a complete genome sequence of the bacterium to aid in the development of vaccines and methods for preventing fish streptococcosis and zoonotic infections i...

Channel catfish (Ictalurus punctatus) is an important agricultural fish that has been plagued by Streptococcus iniae (S. iniae) infections in recent years, some of them severe. C5a peptidase is an important virulent factor of S. iniae. In this study, the subunit vaccine containing the truncated part of C5a peptidase (pSCPI) was mixed with aluminum hydroxide gel (AH), propolis adjuvant (PA), and...

The presence of lactate oxidase was examined in eight Streptococcus species and some related species of bacteria. A clone (pGR002) was isolated from a genomic library of Streptococcus iniae generated in Escherichia coli, containing a DNA fragment spanning two genes designated lctO and lctP. We show that these genes are likely to be involved in the L-lactic acid aerobic metabolism of this organi...

  In the present study, the effect of the high and low virulence S. iniae strains on oxidative activity and cytotoxic effects of trout spleen macrophages were studied. Spleen macrophages were collected from trout. Luminol was used as chemiluminigenic probe. Phorbolmyristate acetate (PMA) also was used as positive control activating agent of respiratory burst and significantly stimulated pr...

This study evaluated the potential to orally deliver a previously developed Streptococcus iniae vaccine in tilapia using OraljectTM technology. This technology was developed to administer bioactive compounds to monogastric animals, and has been shown to be effective for delivery of a variety of antigens in numerous fish species. Two different formulations containing two doses of vaccine (four t...

We conducted a field trial to evaluate the effectiveness of Aquaflor (50% florfenicol) for controlling mortality associated with Streptococcus iniae in freshwater-reared subadult sunshine bass (female white bass Morone chrysops X male striped bass M. saxatilis). Bacterial samples collected from moribund fish representing a reference population were presumptively identified microbiologically and...

background: strepotococcosis caused by streptococcus iniae is one of the important emerging bacterial diseases in aquaculture sector worldwide. objectives: in this study, the genetic diversity of s. iniae strains was assessed in some rainbow trout farms in iran. methods: gram positive and catalase negative bacterial isolates were first obtained from 100 trout fish farms in 8 states using routin...

Vaccination is a potential approach for prevention and control of disease in fish. The use of genetically engineered vaccines is an effective method and a green intervention to control bacterial infection in aquaculture. However, efforts to develop these vaccines are limited by the lack of conserved protective antigens. In this study, three candidate immunogens (Srr, NeuA, and Hsp) of the patho...

Streptococcus iniae is one of the few Gram positive streptococcal species capable of hydrolyzing starch. The starch hydrolysis test is essential for its identification. The biochemical reaction for starch in multi-test systems (API 20 Strep and API CH 50) is starch acidification, not starch hydrolysis. Furthermore, API systems do not contain S. iniae in their databases, yet fish disease researc...

In Taiwan, the mushroom Antrodia camphorata (AC) is used for medicinal purposes, including cancer prevention and treatment. This study aimed to investigate effect of AC-supplemented diets on innate immunity disease resistance orange-spotted grouper Epinephelus coioides against two bacterial infections—Vibrio alginolyticus Streptococcus iniae. Orange-spotted groupers were fed at 0 (control), 1.5...