I. 15NH4C1 was continuously infused for periods of 120-216 h into the rumens of sheep which were allowed to feed 2 out of every 10 min. These treatments achieved steady metabolic states and allowed the assessment of nitrogen conversions by means of tracer methodology. The sheep were given either a barley diet or one of three hay diets. In two trials, the flow of abomasal material was determined...

Introduction T h e first axiom of ruminology appears to be: ‘The rumen is a strictly anaerobic system’ (Hungate, 1966) but before we accept this we should examine the evidence for and against this assertion. Probably the strongest evidence for anaerobiosis comes from the studies of the reactions in the rumen, from the stoichiometries observed and from the low oxidation-reduction potential obtai...

Introduction: Sulfur is an important component of many functions in the body and is an essential nutrient for beef cattle. It is an important part of the amino acids methionine, cysteine, and cystine. The B-vitamins thiamine and biotin also contain sulfur. Rumen microbes require sulfur for their normal growth and metabolism. A large portion of the sulfur found in typical feedlot diets is a comp...

The objective of this paper was to review recent studies on nutrient synchrony and the effects of synchronization of energy and N supply in the rumen on nitrogen utilization and animal performance. Theoretically, synchronization of energy and N supply in the rumen should allow more efficient use of nutrients by rumen microbes, increase microbial protein and fermentation end products, and thus i...

Ruminant phosphorus excretion and metabolism were studied through a database. Faecal endogenous phosphorus is the main pathway of phosphorus excretion and averages 0.85 of total faecal phosphorus. The remaining 0.15 is unabsorbed dietary phosphorus. Faecal endogenous phosphorus is mainly unabsorbed phosphorus, with saliva being the major source, and is correlated to factors influencing saliva s...

The ability of rumen microorganisms to use fibrous plant matter plays an important role in ruminant animals; however, little information about rumen colonization by microbial populations after weaning has been reported. In this study, high-throughput sequencing was used to investigate the establishment of this microbial population in 80 to 110-day-old goats. Illumina sequencing of goat rumen sa...

One of the potential alternative feed ingredients as animal is water hyacinth. However, low quality and palatability cause hyacinth not being liked by ruminants. way to improve using controlled technology involving rumen microbes. The purpose study was determine level addition molasses fermentation time in improving microbes through test parameters for crude protein fiber content. This used a c...

PCR and real-time PCR primers for the 18S rRNA gene of rumen protozoa (Entodinium and Dasytricha spp.) were designed, and their specificities were tested against a range of rumen microbes and protozoal groups. External standards were prepared from DNA extracts of a rumen matrix containing known numbers and species of protozoa. The efficiency of PCR (epsilon) was calculated following amplificati...

A fed-batch, anaerobic culture system was developed to assess the behavior of Escherichia coli O157:H7 in a rumen-like environment. Fermentation medium consisted of either 50% (vol/vol) raw or sterile rumen fluid and 50% phosphate buffer. Additional rumen fluid was added twice per day, and samples were removed three times per day to simulate the exiting of digesta and microbes from the rumen en...