Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates. While most genera of gut fungi form extensive rhizoidal networks, which likely participate in mechanical disruption of plant cell walls, fungi within the Caecomyces genus do not possess these rhizoids. Here, we describe a novel fungal isolate,...

Molecular microbial ecology techniques are widely used to study the composition of the rumen microbiota and to increase understanding of the roles they play. Therefore, sampling and DNA extraction methods that result in adequate yields of microbial DNA that also accurately represents the microbial community are crucial. Fifteen different methods were used to extract DNA from cow and sheep rumen...

A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of ingesta) of lactate-utilizing bacteria to be made with a high degree of specificit...

the aim of this study was to determine effects of different fatty acid profiles on rumen digestion coefficients, some of rumen metabolism parameters and rumen microbial populations. different oils were used as source of fatty acids such as, oleic, linoleic, linolenic, dha and epa. palmitic acid supplemented from a commercial product (bergafat t-300) containing high palmitic acid percentage. res...

The metabolomic profile of the anaerobic fungus Piromyces sp. F1, isolated from the rumen of goats, and how this is affected by the presence of naturally associated methanogens, was analyzed by nuclear magnetic resonance spectroscopy. The major metabolites in the fungal monoculture were formate, lactate, ethanol, acetate, succinate, sugars/amino acids and α-ketoglutarate, whereas the co-culture...

CON TENT S Fimbriae and Haemagglutinating Activity in Strains of Protezcs humeri BY W. I. H. SHEDDEN . Aberrant Forms of Mycobacterium phlei Produccd by Streptomycin and thcir Multiplication on Streptomycin-frec Media BY S. E. JUHASZ . . . . . . . . . . Rate of Growth of Bacillus cereus between Divisicns BY J. I?. COLLINS and M. H. RICHMOND. . Chemical Composition and Antigenic Structure of Cel...