This paper discusses a vision, motivation, and objectives for a new engineering technology based on the Rosetta System Level Design Language (SLDL) that we call “requirements modeling”. Requirements modeling is what systems engineers and systems analysts do with Rosetta SLDL. Rosetta SLDL is information technology that supports the specification of multi-technology, multifaceted, multi-discipli...

An increasingly used parameter in structural biology is the measurement of distances between spin labels bound to a protein. One limitation to these measurements is the unknown position of the spin label relative to the protein backbone. To overcome this drawback, we introduce a rotamer library of the methanethiosulfonate spin label (MTSSL) into the protein modeling program Rosetta. Spin label ...

MOTIVATION Our focus has been on detecting topological properties that are rare in real proteins, but occur more frequently in models generated by protein structure prediction methods such as Rosetta. We previously created the Knotfind algorithm, successfully decreasing the frequency of knotted Rosetta models during CASP6. We observed an additional class of knot-like loops that appeared to be e...

We present the results of a molecular survey comet 67P/Churyumov-Gerasimenko undertaken with Institut de RadioAstronomie Millimétrique (IRAM) 30-m radio telescope in November–December 2021, when it had its most favourable apparition decades. Observations at IRAM during 12–16 November period covered 8 GHz bandwidth 3 mm, 16 2 and 60 1 mm window domain. These were completed by snapshots on 12–13 ...

Temperature-sensitive (ts) mutations are mutations that exhibit a mutant phenotype at high or low temperatures and a wild-type phenotype at normal temperature. Temperature-sensitive mutants are valuable tools for geneticists, particularly in the study of essential genes. However, finding ts mutations typically relies on generating and screening many thousands of mutations, which is an expensive...

Pairs of genes that function together in a pathway or cellular system can sometimes be found fused together in another organism as a Rosetta Stone protein--a fusion protein whose separate domains are homologous to the two functionally-related proteins. The finding of such a Rosetta Stone protein allows the prediction of a functional linkage between the component proteins. The significance of th...

With the expansion of computer network there is a challenge to compete with the intruders who can easily break into the system. So it becomes a necessity to device systems or algorithms that can not only detect intrusion but can also improve the detection rate. In this paper we propose an intrusion detection system that uses rough set theory for feature selection, which is extraction of relevan...

Space mission planning/scheduling is determining the set of spacecraft activities to meet mission objectives while respecting mission constraints. One important type of mission constraint is data management. As the spacecraft acquires data via its scientific instruments, it must store the data onboard until it is able to downlink it to ground communications stations. Because onboard storage is ...

The Rosetta de novo enzyme design protocol has been used to design enzyme catalysts for a variety of chemical reactions, and in principle can be applied to any arbitrary chemical reaction of interest. The process has four stages: 1) choice of a catalytic mechanism and corresponding minimal model active site, 2) identification of sites in a set of scaffold proteins where this minimal active site...