Micropropagation of Vaccinium spp. using Murashige Skoog (MS) and benzylaminopurine (BAP) has been successfully developed. This work aimed to determine the effect different types substrates auxins (naftalen acetic acid NAA, indol IAA y, butyric IBA) on ex vitro establishment rooting nodal segments V. corymbosum Var. Biloxi create a donor bank blueberry plants. The influence five three concentra...

The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 μM NAA and 4.44 μM BAP. The...

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been developed with significant organogenic response from rooted hypocotyls, hypocotyl segments, and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg L thidiazuron and 0.25% coconut water or 7.0 mg L N-Benzyladenine and 0.1 mg L a-naphthalene acetic acid. Thidiazuron in combinat...

The effects of major mineral salts, L-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (Phoenix dactylifera L.) cv. Mejhoul were evaluated. Different concentrations of ammonium nitrate (NH4NO3; 550, 825 or 1650 mg/L), potassium nitrate (KNO3; 633.3, 950 or 1900 mg/L), calcium chloride dehydrate (CaCl2·2H2O; 147, 220 or 440 mg/L), potassium dihydrogen phosphate (...

Grass pea (Lathyrus sativus L.) is highly nutritious and protein rich legume crop; however, presence of ODAP (β-NOxalyl-L-α, β-diaminopropionic acid) has restricted its use for human and animal consumption. There is need for accelerated breeding of low or zero ODAP cultivars to ensure safer and wider use of grass pea through conventional and biotechnological approaches. The study investigated s...

The current study envisages producing Cordyline plants on a large scale employing costeffective In vitro protocols. The effect of various concentrations and types of plant growth regulators were observed to optimize the efficient media for propagation. Axillary buds excised from the greenhouse grown mother plants of Cordyline terminalis were cultured on MS medium supplemented with BAP (1.0-2.0 ...

The present study aimed to develop a micropropagation protocol for Cadaman (P. persica x P. davidiana) and Garnem dulcis) peach rootstocks. Stem nodal segments with one axillary bud used as explants. MS medium supplemented different concentrations of BA or without IBA was studied determine the best  multiplication stage,  results revealed that 2.0 mg/l BA+0.1 optimal shoot proliferation rootsto...

This study aimed to investigate the presence of beneficial microorganisms in micropropagation Pyrus communis rootstocks through isolation, identification, and selection auxin-producing bacteria. The selected were also inoculated vitro evaluate their effect on plant rooting process behavior during acclimatization phase. results showed endophytic tissue cultures ‘OH×F87′ ‘PDW’ selections. A low d...

To evaluate the effect of plastic film coverage on ex vitro acclimatization arrow cane (Gynerium sagitatum Aubl.), shoots “Criolla”, Martinera” and “Costera” cultivars were in micropropagated a medium supplied with 6-Benzylaminopurine half them rooted 1-Naphtalene acetic acid. Rooted unrooted transplanted shade house fog irrigation, into trays (72 clusters per tray), using peat as substrate was...