Although inhibition of RNA interference (RNAi) by plant virus proteins has been shown to enhance viral replication and pathogenesis in plants, no viral gene product has as yet been shown to inhibit RNAi in vertebrate cells. Here, we present evidence demonstrating that the highly structured approximately 160-nucleotide adenoviral VA1 noncoding RNA can inhibit RNAi at physiological levels of expr...

Invertebrate RNA viruses are targets of the host RNA interference (RNAi) pathway, which limits virus infection by degrading viral RNA substrates. Several insect RNA viruses encode suppressor proteins to counteract this antiviral response. We recently demonstrated that the dsDNA virus Invertebrate iridescent virus 6 (IIV-6) induces an RNAi response in Drosophila. Here, we show that RNAi is suppr...

Expression of insect-targeted RNA interference (RNAi) constructs in transgenic plants is a promising approach for agricultural pest control. Compared to conventional chemical insecticides, RNAi target specificity high and the potential negative environmental effects low. However, although numerous laboratory studies show insect growth inhibition by double stranded or artificial microRNA, few th...

The discovery of RNA interference (RNAi) in C. elegans has had a major impact on scientific research, led to the rapid development of RNAi tools and has inspired RNA-based therapeutics. Astonishingly, nematodes, planaria and many insects take up double-stranded RNA (dsRNA) from their environment to elicit RNAi; the biological function of this mechanism is unclear. Recently, the E. coli OxyS non...

As ribonucleic acid (RNA) nanotechnology has advanced, it has been applied widely in RNA-based therapeutics. Among the range of approaches, enzymatically synthesized RNA structures for inducing RNA interference in cancer cells have potential for silencing genes in a target-specific manner. On the other hand, the efficiency of gene silencing needs to be improved to utilize the RNA-based system f...

When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitiv...

objective: rna interference (rnai) is the most potent technique for gene silencing in eukaryotic cellular system at transcriptomic level. genetic disorders and cancers are important targets for therapeutic development of this technique. in order to bypass the temporary dpwnregulation by sirna, a new generation of shrna named shrnamir has developed. silencing construct with structure similar to ...

Introduction. Sustainable farming requires new tools for the control of pathogens, since there is a constant evolution to overcome current biological and chemical strategies. The information provided by transcriptomics allows creating possibilities tackle pathogens. It possible interrupt genetic expression pathogen disable it using RNA interference (RNAi). Objective. To perform an analysis emer...

Telomeres are protective structures present at the ends of linear chromosomes and consist of simple repeating-DNA sequences and specialized proteins [1, 2]. Integrity of the telomeres is important in maintaining genome stability[1-6]. RNA interference(RNAi) involves short double-stranded RNA (21-23 nucleotides long), termed short interference RNA(siRNA), resulting in the downregulation of genes...