Reactive hyperemia (RH) in forearm muscle or skin microcirculation has been considered as a surrogate endpoint in clinical studies of cardiovascular disease. We evaluated two potential confounders that might limit such use of RH, namely laterality of measurement and intake of nonsteroidal anti-inflammatory drugs (NSAIDS). 23 young non-smoking healthy adults were enrolled. In Experiment 1 (n=16)...

Reactive hyperemia (RH) in forearm muscle or skin microcirculation has been considered as a surrogate endpoint in clinical studies of cardiovascular disease. We evaluated two potential confounders that might limit such use of RH, namely laterality of measurement and intake of non-steroidal anti-inflammatory drugs (NSAIDS). Twenty-three young non-smoking healthy adults were enrolled. In Experime...

the immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of toxoplasma gondii (rh strain) was investigated. immunized mice survived challenge infection and displayed increased interferon gamma (inf-γ ) production. on the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to concanavalin a (con a). no tachyz...

We tested the virulence in mice of Toxoplasma gondii RH strain tachyzoites containing various copies of the chloramphenicol acetyl transferase-herpes simplex virus thymidine kinase fusion sequence (CAT-HSTK). Tachyzoite isolates containing >/=five copies of the fusion sequence were not lethal to female CD-1 outbred or BALB/c inbred mice, at doses up to 10(6) parasites, while the parental RH str...

Thermoplastic sugar beet pulp (thermo-mechanical processing was discussed in previous studies) was formed into film strips by extrusion. Film tensile properties are discussed according to the molecular structure of external plasticizer. Sorbitol, fructose and adipic acid have a marked antiplasticizing effect, while urea and xylitol gave higher ultimate tensile stress than glycerol for a compara...

The Beverley strain of Toxoplasma gondii produces a chronic infection in mice and other laboratory animals, causing few or no fatalities. There is an immune response to the parasite that can be detected by a variety of serological teclmiques, with however but little understandillg of the significance of the phemmenon. The purposes of these experiments were to determine: 1) the nature of this im...

A series of mono- and bis-salicylaldimine ligands and their corresponding Rh(i) complexes were prepared. The compounds were characterised using standard spectroscopic techniques including NMR, IR spectroscopy and mass spectrometry. The salicylaldimine ligands and complexes were screened for antiparasitic activity against two strains of Plasmodium falciparum i.e. the NF54 CQ-sensitive and K1 CQ-...

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5o and 3o ends of SAG2, respectively, with...

The aerosol survival of Escherichia coli JEPP, a particularly sensitive strain, has been studied in a nitrogen atmosphere (> 99.9 yo) when sprayed from suspension in solutions of sodium glutamate or glycerol, and collected into phosphate buffer with or without M-sucrose. The response caused by changes in relative humidity (RH) to 30% and I O O ~ ~ before collection was also examined, These two ...

The Toxoplasma gondii population consists of multiple strains, defined by genotype and virulence. Previous studies have established that protective immunity to this organism is mediated by IL-12, which drives T cells to produce IFN-gamma. Paradoxically, although type I and type II strains of T. gondii both induce IL-12 and IFN-gamma in the mouse, type I parasites are lethal, whereas type II str...