Introduction and aim. Mutation(s) in the gene encoding CD19 molecule affect protein expression primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster cheaper than other mutation detection methods, rarely used diagnosis of PID. study aimed to genetically identify deficiency, a PID, using method. Material methods. A total 8 patients two healthy controls were included relevant...

BACKGROUND/AIMS The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS We performed PCR-RFLP to detect and identify Mycobacterium spp....

A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method wa...

Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of locus has been carried out to determine different genotypes T. (strain types I, II, and III) that are associated with human toxoplasmosis. The several linked toxoplasmosis have identified through study locus. In this investigation, PCR-RFLP was found be useful, simple method genotypic cha...

PURPOSE To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of spa gene and to assess the utility of spa genotyping over bacteriophage typing in the discrimination of the strains. MATERIALS AND METHODS Studies were undertaken on 125 MRSA strains representing the mo...

PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and ...