Substrate properties of various morpholinonucleoside triphosphates in the reaction of DNA elongation catalyzed by DNA polymerase beta, reverse transcriptase of human immunodeficiency virus (HIV-1 RT), and reverse transcriptase of Moloney murine leukemia virus (M-MuLV RT) were compared. Morpholinonucleoside triphosphates were utilized by DNA polymerase beta and HIV-1 reverse transcriptase as sub...

The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/C...

The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillu...

List of non-standard abbreviations ATSDR Agency for Toxic Substances and Disease Registry GSK-3β Glycogen synthase kinase-3β Kim-1 Kidney injury molecule-1 RT-PCR Reverse transcriptase polymerase chain reaction

Abstract Rabies is an acute progressive and zoonotic disease which caused by the rabies virus. virus prototype belonging to genus lyssavirus in family Rhabdoviridae of order Mononegavirales. The aim this study was develop a nested reverse-transcriptase polymerase chain reaction (nRTPCR) for detection along with canine internal control monitor integrity extracted RNA. A pair outer inner primer t...

D) DIGESTION WITH Hind III AND EcoRI PROTOCOL, a) cDNA synthesis by Reverse Transcriptase is initiated with oligo(dT) according to Gubler and Hofmann (1) . b) The double stranded cDNA is methylated, and (c) a synthetic CCTTGAATTCAAGCNH oligonucleotlde pGCTTGAATTCAAGC CCAACTTAACTTCCNN is added. This generates a Hindlll recognition sequence (AAGCTT) when ligated to the oligo (dA) track at the 3 t...

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conclusions: this study indicates that rsv is a substantial cause of respiratory tract infection in infants less than 2 years old. rt-pcr offers a rapid method for detection of rsv in hospitalized children with lrti. results: during the study period, 9 children had a positive result for rsv infection. the median age of these patients was 10 months. bronchiolitis was the clinical diagnosis of pa...