materials and methods two-hundred pasteurized (n = 100) and nonpasteurized (n = 100) cheese samples were collected. the initial isolation was performed on pemba specific medium. antibiotic susceptibility testing was performed using several antibiotic disks, according to the guidelines of the clinical laboratory and standards institute. specific primers amplifying the hbla enterotoxin-encoding g...

BACKGROUND In this study, we attempted to screen and investigate antibacterial activity of Bacillus species, which were isolated from conjunctiva, against other eyes pathogens. METHODS To examine predominant isolates of Bacillus subtilis, B. pumilus, B. cereus and B. mojevensis, isolated from conjunctiva for their antimicrobial activity against indicator microorganisms as Micrococcus luteus, ...

The Bacillus cereus group of bacteria comprises soil-dwelling saprophytes but on occasion these bacteria can cause a wide range of diseases in humans, including food poisoning, systemic infections and highly lethal forms of anthrax. While anthrax is almost invariably caused by strains from a single evolutionary lineage, Bacillus anthracis, variation in the virulence properties of strains from o...

objective: bacillus cereus (b. cereus) is a gram-positive, spore-forming bacteria widely distributed in the environment. this bacteria is an opportunistic human pathogen that can cause diarrheal and emetic types of food poisoning. the diarrhea type of food poisoning can be caused by hemolysin bl (hbl), non-hemolytic (nhe), and cytotoxin k enterotoxins. rice is commonly contaminated with b. cere...

The immunoreactivities of hydrogen fluoride (HF)-released cell wall polysaccharides (HF-PSs) from selected Bacillus anthracis and Bacillus cereus strains were compared using antisera against live and killed B. anthracis spores. These antisera bound to the HF-PSs from B. anthracis and from three clinical B. cereus isolates (G9241, 03BB87, and 03BB102) obtained from cases of severe or fatal human...

A polyphasic taxonomic study was performed on seven Bacillus-like bacteria isolated from three hypersaline and alkaline lakes located in China, Kenya and Tanzania. All strains were moderately halophilic and alkaliphilic, Gram positive, motile rods. The DNA G+C content from the seven isolates ranged from 42.2 to 43.4mol% and their major fatty acid was anteiso-C(15:0). Strain CG1(T), selected as ...

Variation among isolates of Bacillus anthracis was examined by using restriction fragmentation patterns and the PCR performed with arbitrary and sequence-specific oligonucleotide primers. The patterns were compared with the patterns generated from strains of closely related species belonging to the "Bacillus cereus group" of bacteria, including B. cereus, Bacillus thuringiensis, and Bacillus my...

Abstract The accumulation of toxic chemical constituents in sludge and wastewater has fuelled an interest investigating efficient eco-friendly remediation approaches. In this study, a set bacterial samples were isolated from petroleum tested for their ability to degrade different aromatic pollutants, including azo dyes emerging pollutants. Although exhibiting differential specificity, all isola...

AIMS To isolate, select and evaluate Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. METHODS AND RESULTS Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibition of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium,...

Bacillus species form an heterogeneous group of Gram-positive bacteria that include members that are disease-causing, biotechnologically-relevant, and can serve as biological research tools. A common feature of Bacillus species is their ability to survive in harsh environmental conditions by formation of resistant endospores. Genes encoding the universal stress protein (USP) domain confer cellu...