Objective(s): MicroRNAs (miRNAs) are considered as powerful, post-transcriptional regulators of gene expression in hepatocellular carcinoma cells (HCC). However, the function of miR-92a is still unclear in HCC. Materials and Methods: Expression of miR-92a in human HCC cell lines was evaluated using qRT-PCR. MTT assay and transwell assay were used to examine the function of miR-92a in HepG2 and ...

mGluR1 antagonists have been postulated to be novel CNS drugs, including antipsychotics. Toward this end, the authors developed a beta-lactamase reporter assay to identify mGluR1 antagonists. beta-Lactamase has several interesting features for high-throughput screening, including very high sensitivity and less well-to-well variation than other reporter enzymes. mGluR1-expressing Chinese hamster...

Abstract Background Our previous study shows that LINC01278 inhibits the malignant proliferation and invasion of papillary thyroid carcinoma (PTC) cells by regulating miR-376c-3p/DNM3 axis. However, regulation mechanism expression in PTC is still unclear. Methods The luciferase reporter ChIP assays were used to confirm binding LEF-1 putative promoter site gene. RNA immunoprecipitation pulldown ...

Although transgene-based reporter gene assays have been used to discover small molecules targeting expression of cancer-driving genes, the success is limited due to the fact that reporter gene expression regulated by incomplete cis-acting elements and foreign epigenetic environments does not faithfully reproduce chemical responses of endogenous genes. Here, we present an internal ribosome entry...

Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, stenosis, microvascular obstruction heart failure, it is particularly important seek new strategies mitigate reperfusion injury. In this paper, we will investigate whether atorvastatin can alleviate ischemia-reperfusion verify its molecul...

Transporters of the ATP-binding cassette (ABC) family such as MDR1 play a pivotal role in persistence of brain homeostasis by contributing to the strict permeability properties of the blood-brain barrier. This barrier on one hand compromises treatment of central nervous system diseases by restricting access of drugs; on the other hand, an impaired or altered function of barrier building cells h...

The endocrine-disrupting equivalents in effluents from three chemical industry wastewater treatment systems in the vicinity of Yangtze River were determined by several transactivation reporter gene assays. Transient transfections of African green monkey kidney cell line (CV-1) were used to determine the estrogenic, anti-androgenic and anti-thyroid equivalents in the effluents. Organic extracts ...