A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

before being an islamic thinker, saied qutb was a literary scholar and critic whose impressionistic character as an islamic thinker was partially because of his magic and eloquent expression. nonetheless many people still know him as an islamic thinker without being informed of his critical and literary approaches, so his works on criticism and literature are becoming forgotten. this paper is a...

for centuries, the famous iranian mystic and poet, mowlana, has had a great influence on the literature of different nations through his mystical frenzy. poets and writers of other countries including spain, have been enamoured by his poems as well as his literary works. poets who have been influenced by the thoughts of this famous persian mystic have thus been trained in this area. this claim ...