Pleurotus eous colonies regenerated on plates containing complete medium overlaid with soft agar gave 0.28% regeneration efficiency, while that of Pleurotus flabellatus was slightly less (0.24%). In PEG (polyethylene glycol) induced fusion experiments with Pleurotus eous and Pleurotus flabellatus, 0.18% of fusion frequency was achieved and four fusant colonies were recovered. The fusant No.4 co...

The present study was undertaken to investigate the possibility for developing Efficient Plant regeneration protocol in Vigno mungo L. For this, the effect of Cytokinens on in vitro regeneration from Cotyledonary explants was studied in Black gram (Vigno mungo L.) Hepper Var Vamban-1. The cytokinins used were 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and kinetin (Kn). The regenerated shoots...

using haploid technique in breeding of sugar beet as an allogamous plant will facilitate breeding efforts. in this method,a pure line with a desirable genotype can be achieved by unfertilized ovule culture. therefore ovule culture was done from populations with numbers: 27621, 27624, 27626 and 27629. young flowering stems were collected and ovules were isolated from the flower buds after steril...

An experiment was conducted to study the effect of growth regulators on callus induction in rice embryo culture. In this experiment, caullogenesis was initiated from the matured seeds of seven indica rice varieties viz., TRY 1, TRY 2, Pokkali, CSR 10, W.Ponni, BPT 5204 and IR 29. The medium used for this callus induction was Murashige and Skoog (1962) commonly referred as MS medium with six dif...

The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...

Cucumis melo L. is one of the most economically important horticultural crops worldwide. However,   low plant regeneration frequency of this plant during genetic transformation is  the  major hurdle for  applying biotechnological approaches. Hence, this study aimed to evaluate the effect of 6-benzyladenine (BA), cefotaxime (CTX), kanamycin (KAN), and indole-3-acetic acid (IAA) on the regenerati...

Sorghum is a wonder crop from physiological point of view. It is the most important cereal crop, after rice and wheat.  The number of reports describing the use of transgenic Sorghum for basic studies in Biotechnology is still limited when compared to other crops. In one hand, the success of the transformation techniques is mainly dependent upon the availability of optimal protocols for highly ...

Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from ...