نتایج جستجو برای: reconstitution

تعداد نتایج: 12512  

Journal: :Plant & cell physiology 2007
Akira Nozawa Hideaki Nanamiya Takuji Miyata Nicole Linka Yaeta Endo Andreas P M Weber Yuzuru Tozawa

We describe here a novel proteoliposome reconstitution system for functional analysis of plant membrane transporters that is based on a modified wheat germ cell-free translation system. We established optimized conditions for the reconstitution system with Arabidopsis thaliana phosphoenolpyruvate/phosphate translocator 1 (AtPPT1) as a model transporter. A high activity of AtPPT1 was achieved by...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1975
V G Moore R E Atchison G Thomas M Moran H F Noller

Extraction with 2 M lithium chloride removes a group of proteins (LiC1 SP) from 50S ribosomal subunits. Both the LiC1 SP and the resulting cores, which contain the remaining proteins as well as 5S and 23S RNA, lack peptidyl transferase activity, as measured by the "fragment reaction". Activity can be restored to the LiC1 cores by reconstitution with LiC1 SP under conditions of high temperature ...

Journal: :Sexually Transmitted Infections 2000

2009
Douchi Matsuba Takako Terui Jin O-Uchi Hiroyuki Tanaka Takao Ojima Iwao Ohtsuki Shin'ichi Ishiwata Satoshi Kurihara Norio Fukuda

Protein kinase A (PKA)-dependent phosphorylation of troponin (Tn)I represents a major physiological mechanism during beta-adrenergic stimulation in myocardium for the reduction of myofibrillar Ca2+ sensitivity via weakening of the interaction with TnC. By taking advantage of thin filament reconstitution, we directly investigated whether or not PKA-dependent phosphorylation of cardiac TnI (cTnI)...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1979
S Weisbrod H Weintraub

The globin gene is preferentially sensitive to digestion by DNase I in erythrocyte chromatin but not in brain, fibroblast, or oviduct chromatin. Elution of the erythrocyte chromatin with 0.35 M NaCl leads to no detectable change in the gross structure of individual nucleosomes; however, in this depleted chromatin the globin gene is no longer preferentially sensitive to DNase I. Reconstitution o...

2015
Ighovwerha Ofotokun Kehmia Titanji Tatyana Vikulina Susanne Roser-Page Masayoshi Yamaguchi Majd Zayzafoon Ifor R. Williams M. Neale Weitzmann

HIV infection causes bone loss. We previously reported that immunosuppression-mediated B-cell production of receptor activator of NF-κB ligand (RANKL) coupled with decline in osteoprotegerin correlate with decreased bone mineral density (BMD) in untreated HIV infection. Paradoxically, antiretroviral therapy (ART) worsens bone loss although existing data suggest that such loss is largely indepen...

Journal: :Blood 2015
Alessandra Roberto Luca Castagna Veronica Zanon Stefania Bramanti Roberto Crocchiolo James E McLaren Sara Gandolfi Paolo Tentorio Barbara Sarina Inna Timofeeva Armando Santoro Carmelo Carlo-Stella Benedetto Bruno Cristiana Carniti Paolo Corradini Emma Gostick Kristin Ladell David A Price Mario Roederer Domenico Mavilio Enrico Lugli

Early T-cell reconstitution following allogeneic transplantation depends on the persistence and function of T cells that are adoptively transferred with the graft. Posttransplant cyclophosphamide (pt-Cy) effectively prevents alloreactive responses from unmanipulated grafts, but its effect on subsequent immune reconstitution remains undetermined. Here, we show that T memory stem cells (TSCM), wh...

2016
Ryan M. Broxterman Phillip F. Skiba Jesse C. Craig Samuel L. Wilcox Carl J. Ade Thomas J. Barstow

The sustainable duration of severe intensity exercise is well-predicted by critical power (CP) and the curvature constant (W'). The development of the W'BAL model allows for the pattern of W' expenditure and reconstitution to be characterized and this model has been applied to intermittent exercise protocols. The purpose of this investigation was to assess the influence of relaxation phase dura...

Journal: :Blood 2006
José A Borghans Robbert G Bredius Mette D Hazenberg Helene Roelofs Els C Jol-van der Zijde Jeroen Heidt Sigrid A Otto Taco W Kuijpers Willem E Fibbe Jaak M Vossen Frank Miedema Maarten J van Tol

The immune system of patients with severe combined immunodeficiency (SCID) reconstitutes to a large extent during the first years after hematopoietic stem cell transplantation (HSCT). It was suggested, however, that accelerated loss of thymus output may cause impaired immune function at the long term. To address this issue, we studied patients with SCID who underwent allogeneic HSCT 5 to 32 yea...

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