Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...

alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. in the present study, alkaline phosphatase gene was inserted into the pet15b vector. the recombinant dna was then expressed using iptg as an inducer. the expression of this enzyme was optimized by changing expression conditions...

This paper outlines the immobilization of recombinant dimeric unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). The enzyme was quite stable (remaining unaltered its activity after 35 h at 47 °C and pH 7.0). Phosphate destabilized enzyme, while glycerol stabilized it. not immobilized on glyoxyl-agarose supports, it albeit in inactive form vinyl-sulfone-activated supports. rAaeUPO glutara...

An original 1-acetato-4-(1-pyrenyl)-1,4,7-triazacyclononane (AcPyTACN) was synthesized for the immobilization of a His-tagged recombinant CODH from Rhodospirillum rubrum (RrCODH) on carbon-nanotube electrodes. The strong binding enzyme at Ni-AcPyTACN complex affords high current density 4.9 mA cm−2 towards electroenzymatic CO2 reduction and stability more than 6×106 TON when integrated gas-diff...

Lignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of for environmental prevention and governance was promoted by exploring effects small molecule substances on activities. In this study, (LiP) gene from Irpex Lacteus heterologous expressed Escherichia coli. The activity recombinant LiP 11.2 U/mg, optimum temperature 37℃, pH 3.5. range 20-45℃ ...

OBJECTIVE To construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2. METHOD Primers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ...

Background: Recombinant insulin glargine, a long-acting analogue of insulin, is expressed as proinsulin in host cell and after purification and refolding steps cleaved to active insulin by enzymatic digestion using trypsin and carboxypeptidase B. Since the proinsulin's B and C chains have several internal arginine and lysine residues, a number of impurities are generated following treatment wit...

objective: helicobacter pylori is a widely distributed gram negative bacterium that infects the human stomach and duodenum. some antibiotic regimens are subjected to cure the infection but the cost of drugs, poor patient compliance and emerging of antibiotic-resistant strains are limiting the usefulness of these antibiotic therapies. therefore, interest in developing a h. pylori vaccine is grow...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...