The question of how genetic materials are trafficked in and out of the cell nucleus is a problem of great importance not only for understanding viral infections but also for advancing gene-delivery technology. Here we demonstrate a physical technique that allows gene trafficking to be studied at the single-gene level by combining sensitive fluorescence microscopy with microinjection. As a model...

While Highly Active Antiretroviral Therapy (HAART) has significantly decreased the mortality of human immunodeficiency virus (HIV)-infected patients, emerging drug resistance to approved HIV-1 integrase inhibitors highlights the need to develop new antivirals with novel mechanisms of action. In this study, we screened a library of microbial natural compounds from endophytic fungus Colletotrichu...

The presequence of the ornithine transcarbamylase precursor (pOTC) was fused to green fluorescent protein (GFP), yielding pOTC-GFP and pOTCN-GFP containing the presequence plus 4 and 58 residues of mature ornithine transcarbamylase, respectively. When GFP cDNA was transfected into COS-7 cells, the cytosol and nucleus were fluorescent. On the other hand, pOTC-GFP cDNA gave strong fluorescence of...

This paper presents a distance protection test procedure by applying the Real-Time Digital Simulator (RTDS) of a power system. RTDS is a tool to design, develop, and test power-system protection. The RTDS enables real-time computation of electromagnetic phenomena with a calculation time step of even 50μs. The hardware allows the import and export of many signals from the simulator to an ext...

A large economic literature discusses the implications of export sanctions for a variety of states around the world. This paper investigates the macro-level consequences of imposing oil export barriers on an oil exporting country. We employ a large real financial computable general equilibrium for Iran. The model is calibrated based on 1999 Social Accounting Matrix for the economy of Iran inc...

macroeconomic policies lead to changes in farming system. these changes are accompanied by some most prominent factors that affect soil erosion. change in relative prices and its impact on soil erosion, via induced changes in crop patterns and agricultural practices, are related to policies implemented. one of the major goals of the present study is to emphasize on the flow of trade policies in...

The aim of this paper is to present the definition of the measures for the import-coupling of OCL expressions, along with the empirical validation of these as early indicators of the maintainability of OCL expressions. This empirical validation has been carried out by means of an experiment and its replica, conducted with undergraduate students of Spanish and Argentinean universities, respectiv...

The effects of temperature and light on the breakdown of the coral-Symbiodinium symbiosis are well documented but current understanding of their roles during initial uptake and establishment of symbiosis is limited. In this study, we investigate how temperature and light affect the uptake of the algal symbionts, ITS1 types C1 and D, by juveniles of the broadcast-spawning corals Acropora tenuis ...

abstract the present research accomplished to determination of the trade liberalization effects on import function of iran’s wheat crop using time series data from 1357 to 1387 that earn from sources such as agricultural and aerology statistics annual letter, customhouse and iran central bank website.in this investigation, at first, wheat liberalization index was measured by using level of inte...