Of the membrane proteins of known structure, we found that a remarkable 67% of the water soluble domains are structurally similar to water soluble proteins of known structure. Moreover, 41% of known water soluble protein structures share a domain with an already known membrane protein structure. We also found that functional residues are frequently conserved between extramembrane domains of mem...

Dietary non-heme iron contains ferrous [Fe(II)] and ferric [Fe(III)] iron fractions and the latter should hydrolyze, forming Fe(III) oxo-hydroxide particles, on passing from the acidic stomach to less acidic duodenum. Using conditions to mimic the in vivo hydrolytic environment we confirmed the formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues of these (~ 10 nm hyd...

Mitochondrial porin, the outer membrane pore-forming protein, was isolated in the presence of detergents and converted into a water-soluble form. This water-soluble porin existed under nondenaturing conditions as a mixture of dimers and oligomers. The proportion of dimers increased with decreasing porin concentration during conversion. Water-soluble porin inserted spontaneously into artificial ...

two newly-created water-soluble complexes of palladium(ii)/platinum(ii)-dithiocarbamate, [pd/pt(phen)(pyr-dtc)]no3 (phen = 1,10-phenanthroline and pyr-dtc = pyrrolidinedithiocarbamate) were synthesized. also, their chemical characteristics are reported in the current research paper. in these complexes, the dithiocarbamato ligand coordinates to pt(ii) or pd(ii) center with two sulfur atoms as bi...

Two novel, water-soluble, merocyanine fluorophores were readily prepared by microwave-assisted synthesis. Full optical characterization was performed in a series of protic and aprotic solvents, the dyes displayed fluorescence red region with up to 20-fold decrease brightness water, demonstrating strong environmental sensitivity hereby termed as solvato-fluorogenicity (to distinguish from solvat...

The affinity chromatographic separation procedure was tested for its utility in quantitating the affinity of proteins to both insolubilized ligand and corresponding competing soluble ligand. An expression has been derived that (1) allows the determination of binding constants for the interaction of a soluble protein species with an affinity chromatography matrix involving active site binding, (...

Owing to the requirement of surgical senior house officers to prioritise acute admissions in the accident and emergency department, they are increasingly unable to be present in theatre to assist the surgical registrar. As a consequence, the surgeon has to rely on the scrub team to provide much-needed retraction with cases such as open appendicectomies. We have found the use of the Alexis® woun...

The immobilization and mineralization processes carried out by soil microorganisms have a significant role in controlling the availability of phosphorus soil. A class helpful known as phosphate solubilizing microbes are able to hydrolyze insoluble organic inorganic compounds into soluble P (phosphorus) form that is readily taken plants. objective this study was evaluate different waste Aspergil...

It was shown in preceding papers (2, 3) that when 3,4-benzpyrene dissolved in benzene was irradiated with monochromatic radiation of wave length 2,537 A. in the presence of oxygen, at least two water-soluble substances were included in the products and that these could be recognized, and some of their properties deduced, by spectrophotometric methods. The first, substance A, was characterized a...

Information regarding transferrin (Tf) receptor degradation is largely incomplete. HL60 cells were shown to release to their growth medium a Tf-binding protein which could be immunoprecipitated by anti-Tf receptor monoclonal antibodies (MoAbs) B3/25 and OKT9. Soluble Tf receptor was detected in the medium within one hour of replating of cells, and its release was inhibited at 4 degrees C. The a...