نتایج جستجو برای: rbcl sequence

تعداد نتایج: 406813  

Journal: :Acta biochimica et biophysica Sinica 2004
Peng-Da Ma Tian-Cheng Lu Xiao-Fu Zhou Xiao-Juan Zhu Xing-Zhi Wang

Spinach Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) large (rbcL) and small (rbcS) subunits were separated by SDS-PAGE, and protein amount and purity were determined by Bradford assay. Polyclonal antibodies against rbcL and rbcS subunit were generated in female BALB/c mice and had no cross-reaction with each other. A total of 81 microg antigens were used and 0.3 ml anti-sera with t...

2015
Paul B. Hamilton Keely E. Lefebvre Roger D. Bull

Single cell Chelex® DNA extraction and nested PCR amplification were used to examine partial gene sequences from natural diatom populations for taxonomic and phylogenetic studies at and above the level of species. DNA was extracted from cells that were either fresh collected or stored in RNAlater. Extractions from Lugol's fixation were also attempted with limited success. Three partial gene seq...

1998
Tzen-Yuh Chiang Barbara A. Schaal Ching-I Peng

Universal primers for amplifying and sequencing a noncoding spacer between the atpB and rbcL genes of the chloroplast DNA were constructed from the published sequences of Marchantia (a liverwort), tobacco, and rice. Our results indicate an evolutionary trend of increasing spacer size from liverworts, through mosses, to vascular plants. This atpB-rbcL spacer is AT-rich, consistent with other chl...

2015
María-Carmen Fernández-Pinos Marta Casado Gemma Caballero Erik R. Zinser Jordi Dachs Benjamin Piña Amanda M Cockshutt

Newly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a ...

Journal: :Biochemical systematics and ecology 2001
van der Bank H Wink Vorster Treutlein Brand van der Bank M Hurter

Phylogenetic relationships between Encephalartos altensteinii Lehmann, E. arenarius R.A. Dyer, E. horridus (Jacquin) Lehmann, E. latifrons Lehmann, E. lehmannii Lehmann, E. longifolius (Jacquin) Lehmann, E. princeps R.A. Dyer and E. trispinosus (Hooker) R.A. Dyer were studied, using E. ferox Bertoloni f. as outgroup. Three continuous and one discontinuous buffer systems were used and gene produ...

2003
Birgitta Bremer Kåre Bremer Mark W. Chase James L. Reveal Douglas E. Soltis Pamela S. Soltis Arne A. Anderberg Michael F. Fay Peter Goldblatt Walter S. Judd Mari Källersjö Jesper Kårehed Kathleen A. Kron Johannes Lundberg Daniel L. Nickrent Richard G. Olmstead Bengt Oxelman Chris Pires James E. Rodman Paula J. Rudall Vincent Savolainen Kenneth J. Sytsma

s. St. Louis: Missouri Botanical Garden, 250. Conti E, Litt A, Sytsma KJ. 1996. Circumscription of Myrtales and their relationships to other rosids: evidence from rbcL sequence data. American Journal of Botany 83: 221–233. Contreras VR, Scogin R, Philbrick CT. 1993. A phytochemical study of selected Podostemaceae: systematic implications. Aliso 13: 513–520. Cronquist A. 1981. An integrated syst...

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