Unlike human plasma, rat plasma was found to contain substantial quantities of alkaline phosphatase. The large amount of phosphatase activity in rat plasma interferes with assay methods that employ orthophosphoric acid esters as substrates or reaction intermediates. Although these methods are effective when applied to human plasma samples, they cannot be used with rodent plasma.

Stent implantation into aortocoronary saphenous vein grafts (SVG) releases particulate debris and soluble vasoactive mediators, for example, serotonin. We now analyzed effects of the soluble mediators released into the coronary arterial blood during stent implantation on vasomotion of isolated rat epicardial coronary artery segments and on coronary flow and left ventricular developed pressure i...

Zinc oxide nanoparticles (ZnO-NPs) are widely used in the food and fertilizer industries. During our previous study, bean fertilized by two doses (20 40 ppm) of ZnO-NPs with a particle size 20 nm was investigated on rats. Therefore, this experiment designed to evaluate effect that were administrated orally rats once day at ppm. Hematological biochemical parameters quantified addition investigat...

Sulfur mustard (HD) is an alkylating agent that reacts rapidly with macromolecular targets resulting in the formation of stable adducts providing depots for markers of exposure. The purpose of this study was to validate an analytical procedure for detection of HD-plasma protein adducts and to establish the utility of the method in an HD rat inhalation study. Calibration curves were prepared in ...

It has been hypothesized that elevated plasma Homocysteine (Hcy) plays a role in the pathogenesis of Alzheimer’s disease (AD) and age-related cognitive decline. The mechanism of Hcy neurotoxicity in the brain is controversial as well Hcy is a ligand of NMDA receptor. Memantine, an uncompetitive antagonist of N-methyl-D-aspartate (NMDA) receptors approved for the treatment of moderate to severe ...

Free vascular endothelial growth factor (VEGF) is undetectable in plasma during human pregnancy. However, studies examining pregnant rats have reported both low (8-29 pg/ml) and high (527-1,030 pg/ml) free VEGF. These discrepancies cast uncertainty over the use of rat models to study angiogenic factors in pregnancy and preeclampsia. This study investigates methodological factors that may explai...