Genetic relationships were studied among four taxa in the genus Chaenomeles using isozyme analysis. The band patterns obtained with six polymorphic isozyme systems provided 108 reliable markers, which were scored as unordered multistate traits. A cluster analysis as well as a multidimensional scaling analysis grouped the taxa in agreement with previously published results obtained with RAPD (ra...

DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...

Genetic relationship of a newly discovered teosinte from Nicaragua, Zea nicaraguensis with waterlogging tolerance, was determined based on randomly amplified polymorphic DNA (RAPD) markers and the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA using 14 accessions from Zea species. RAPD analysis showed that a total of 5,303 fragments were produced by 136 random decamer prim...

The objective of this work is to present an appropriate set of RAPD (random amplified polymorphic DNA) markers using single and multiplex PCR analysis suitable for the characterization of the endemic Cuban species Carica cubensis and the establishment of genetic relationships with the cultivated species Carica papaya. RAPD markers presented a high level of polymorphism. In addition, the incorpo...

genetic diversity of 22 morphotypes of diploid wheat (t. urartu) was investigated using morphological traits and rapd markers. morphological characterizations were carried out based on 17 traits and statistical parameters of dispersion and diversity of agronomic traits were calculated. statistical analysis showed significant differences for all characters among morphotypes. dendrograms were gen...

Trichophyton rubrum is an important cause of dermatomycoses. Molecular strain typing methods have recently been developed to address questions about epidemiology and source of relapse following treatment. This report describes the application of RAPD for molecular strain differentiation of this fungus utilizing the primers 1- (5'-d[GGTGCGGGAA]-3') and 6- (5'-d[CCCGTCAGCA]-3'). A total of five R...

Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different num...

Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of ...

Genetic variations of 15 Brahmi (Bacopa monnieri L.) accessions were evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. During RAPD analysis, amplification of genomic DNA of the 15 accessions by 22 primers generated 197 fragments, of which 187 were polymorphic with an average of 8.95 bands per primer. The amplified products varied in size f...