The random amplified polymorphic DNA (RAPD) technique has been used widely for purposes such as the construction of linkage maps, QTL analysis, evaluation of genetic diversity, and parentage tests. However, some minor bands of RAPD display a low reproducibility and low reliability. We describe a simple and efficient method for making minor RAPD bands clearer. The first step of this method is to...

The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five ni...

This study was conducted to determine whether there was any genetic heterogeneity among Helicobacter pylori strains isolated from the antrum and corpus of the same individual in a Malaysian population and to determine the presence of heterogeneous susceptibility of the isolates by comparing PCR-RAPD and antibiotic profiles. Forty-four H. pylori isolates cultured from the antrum and corpus of 22...

Nosocomial infections caused by Staphylococcus aureus are clinically serious and control of such infections requires strain typing to identify the source of contamination. Recently, pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) assay have been introduced and have provided a high level of strain discrimination of S. aureus isolated from clinical specimens. T...

The technique of random amplified polymorphic DNA (RAPD) was adapted and optimized to study Haemophilus ducreyi isolates. A panel of 43 strains isolated from chancroid patients from different countries in Africa, Europe, North America, and Asia were characterized. The strains were also studied with respect to lipooligosaccharide (LOS) migration and immunoblotting patterns and the presence of cy...

1 Introduction The first step of RAPD fingerprinting is the preparation of the target DNA template. Intuitively, minimal DNA template preparation should be necessary for RAPDs since, theoretically, PCR may amplify a single DNA molecule. It seems one would simply homogenize tissue and allow the PCR to "find" and amplify the target DNA. Indeed, many rapid DNA isolation methods designed for use wi...

Seven dextran-producing Leuconostoc strains were differentiated by using a modified randomly amplified polymorphic DNA (RAPD) protocol that incorporated specific primers designed from conserved regions of dextransucrase genes. RAPD profiles showed intraspecies differences among the Leuconostoc mesenteroides strains tested. This modified RAPD protocol will aid in the differentiation of polymer-p...

PURPOSE To assess the amount of structural loss (retinal nerve fiber layer [RNFL] thickness loss, macular thickness [MT] and volume [MV] measured by optical coherence tomography [OCT]) and functional loss (visual acuity [VA], visual field mean deviation [MD], brightness sensitivity, and red perception) necessary for a relative afferent pupillary defect (RAPD) to manifest in patients with glauco...

Isoenzyme and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetics of geographic variation among population samples of Ascaris suum from midwestern localities. Independent estimates of fixation indices (FST) based on isoenzyme and RAPD markers showed the same general patterns of differentiation and substantial statistical correlation (r = 0.70). Of the total es...

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 R...