Assessing the stability of proteins by comparing their unfolding profiles is a very important characterization and quality control step for any biopharmaceutical, and this is usually measured by fluorescence spectroscopy. In this paper we propose Raman spectroscopy as a rapid, noninvasive alternative analytical method and we shall show this has enhanced sensitivity and can therefore reveal very...

A dual-modal approach using Raman spectroscopy and optical pH sensing was investigated to discriminate between normal and cancerous tissues. Raman spectroscopy has demonstrated the potential for in vivo cancer detection. However, Raman spectroscopy has suffered from strong fluorescence background of biological samples and subtle spectral differences between normal and disease tissues. To overco...

The combination of optical trapping with Raman spectroscopy provides a powerful method for the study, characterization, and identification of biological micro-particles. In essence, optical trapping helps to overcome the limitation imposed by the relative inefficiency of the Raman scattering process. This allows Raman spectroscopy to be applied to individual biological particles in air and in l...

The field of medical diagnostics will have tremendous potential gains in the near future due to Raman spectroscopy and microscopy. Applications include surface-enhanced Raman spectroscopy (SERS), signal enhancement targeted to specific analytes, coupling atomic force microscopy (AFM) systems and enabling tip-enhanced Raman spectroscopy (TERS) for nanoscale resolution. These technologies highlig...

A new type of Raman spectroscopy is presented: After transient excitation of molecular modes coherently scattered Raman spectra are investigated in" a delayed probing experiment. The spectral position of the Raman mode is observed after long delay times. The dephasing time is obtained from the time dependence of the scattered amplitudes. Frequency disturbing non-resonant susceptibilities are el...

In this review we describe label-free optical spectroscopy techniques which are able to non-invasively measure the (bio)chemistry in biological systems. Raman spectroscopy uses visible or near-infrared light to measure a spectrum of vibrational bonds in seconds. Coherent anti-Stokes Raman (CARS) microscopy and stimulated Raman loss (SRL) microscopy are orders of magnitude more efficient than Ra...

Raman spectroscopy has become a powerful analytical tool providing detailed and specific information at a molecular level. This benefit together with rapidity and other features of the method yields an advantage of quasi real time monitoring and measuring the structure changes dynamics. There is no doubt that Raman spectroscopy can be ranked among powerful and useful research and analytical met...

introduction raman spectroscopy is a vibrational spectroscopic technique, based on inelastic scattering of monochromatic light. this technique can provide valuable information about biomolecular changes, associated with neoplastic transformation. the purpose of this study was to find raman spectral markers for distinguishing normal samples from cancerous ones in different tissues. materials and...

Total internal reflection (TIR) Raman spectroscopy is an experimentally straightforward, surface-sensitive technique for obtaining chemically specific spectroscopic information from a region within approximately 100-200 nm of a surface. While TIR Raman spectroscopy has long been overshadowed by surface-enhanced Raman scattering, with modern instrumentation TIR Raman spectra can be acquired from...