We have previously shown that the phosphorylation of vitamin B1 to cocarboxylase by an atiozymase preparation is dependent upon the presence of hexosediphosphate and certain heat-stable factors contained in boiled tissue extracts (1). Our results further indicated that this phosphorylation was completely inhibited by 0.0025 to 0.005 M sodium iodoacetate, and that the addition of 0.04 M sodium f...

THE methods employed for the estimation of pyruvic acid are largely based upon the reaction of its carbonyl group with phenylhydrazine. Its estimation in complex biological fluids where there are other compounds which react likewise is therefore difficult and, particularly when pyruvic acid exists in small quantities amidst these compounds, the estimation is unreliable even when controls are ru...

An automated system for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding program. The system includes two high performance liquid chromatography (HPLC) pumps, an autosampler, a column heater, a spectrophotometric detector, and an integrator. With this new method, human error can be eliminated with great repeatability (CV ˆ 0.9%), and we can ru...

A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be qu...

Pyruvic acid is an immunological determinant of the quite rigorously type-specific capsular polysaccharide of pneumococcal type IV (S IV). Removal of pyruvic acid by mild hydrolysis converts the capsular polysaccharide of type IV into an analog of the pneumococcal group-specific C-substance. Depyruvylated S IV resembles C-substance so closely immunologically that it not only precipitates a high...