Abstract Pseudomonas aeruginosa is a bacterium producing industrially utile metabolites, such as rhamnolipids, biopolymers, and pigments. Pyocyanin, the most studied example of pigments, virulence factor that also shows potential for application in, e.g., agriculture, anticancer therapy, energy production. Therefore, inhibitors stimulants pyocyanin production by P. should be studied, nanomateri...

Pseudomonas aeruginosa culture filtrates varied in their ability to slow human ciliary beat frequency (7-71%). This activity did not correlate with known virulence factors. However, a close correlation (r = 0.97) existed between ciliary slowing and pigment content. In a prolonged culture, the increase in activity correlated (r = 0.94) with pigment accumulation. Gel filtration of lyophilized fil...

A biomolecule namely pyocyanin was isolated from Pseudomonas aeruginosa (BTRY1). It was confirmed by UV-Vis spectrum with absorption maxima at 270 nm and was further characterized using Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR). Spectroscopy analyses showed the presence of relevant bonds in their respective structures. Although pyocyanin had antihemolytic, antioxida...

Two virulence factors produced by Pseudomonas aeruginosa are pyocyanin and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12). Pyocyanin damages host cells by generating ROS (reactive oxygen species). 3OC12 is a quorum-sensing signalling molecule which regulates bacterial gene expression and modulates host immune responses. PON2 (paraoxonase-2) is an esterase that inactivates 3OC12 and potentiall...

The transcriptional regulator BrlR from Pseudomonas aeruginosa is a member of the MerR family. Studies have shown BrlR can be activated by the secondary messenger 3′,5′-cyclic diguanylic acid (c-di-GMP) and contributes to P. aeruginosa biofilm tolerance. Previous structural analysis of BrlR-c-di-GMP complex has identified one hydrophobic pocket locating in its C-terminal GyrI-like domain. Howev...

The Pseudomonas aeruginosa-derived phenazine pigments pyocyanin and 1-hydroxyphenazine (1-hp) prime human neutrophils for enhanced, stimulus-activated release of superoxide and myeloperoxidase (MPO), respectively. In the present study, the modulatory potentials of the antimicrobial agents clindamycin, erythromycin, and roxithromycin (10 and 20 micrograms/ml) on the prooxidative interactions of ...

Pseudomonas aeruginosa, is an opportunistic pathogen that causes extensive morbidity and mortality in individuals who are immunocompromised or have underlying medical conditions such as, urinary tract, respiratory tract and skin infections and primarily causes of nosocomial infections [1,2]. Its anon sporulating, gram negative, oxidase positive motile bacterium with a polar flagellum [3], P. ae...

Camellia nitidissima Chi, known as a medicinal and edible plant in China, exhibits multiple bioactivities, especially antibacterial activity. In this study, we investigated the inhibitory effects of the dichloromethane fraction (DF) of C. nitidissima Chi flowers on the pyocyanin production, swarming motility, and swimming motility of Pseudomonas aeruginosa PAO1, at sub-minimum inhibitory concen...

A cost-effective and more rapid means of detection of Pseudomonas aeruginosa in cultures from clinical specimens would be very advantageous. We have developed a modified MacConkey agar (MMA), which enhances pigment production of P. aeruginosa and which, if pyocyanin pigment is present, provides a relatively rapid and very cost-effective identification. The MMA medium inhibits the gram-positive ...