Purine biosynthesis and metabolism, conserved in all living organisms, is essential for cellular energy homeostasis and nucleic acid synthesis. The de novo synthesis of purine precursors is under tight negative feedback regulation mediated by adenosine and guanine nucleotides. We describe a distinct early-onset neurodegenerative condition resulting from mutations in the adenosine monophosphate ...

Presoaking several varieties of seed, especially lettuce, in various 6(substituted) aminoand thiopurine solutions has been found to increase their rate of germination (3, 5, 6). Recently it was also reported that these purine derivatives are synergistic with gibberellin in inducing these biological responses whereas gibberellin alone was only moderately active in stimulating germination of Earl...

A unified synthetic strategy accessing novel 3'-fluorinated purine nucleoside derivatives and their biological evaluation were achieved. Novel 3'-fluorinated analogues were constructed from a common 3'-deoxy-3'-fluororibofuranose intermediate. Employing Suzuki and Stille cross-coupling reactions, fifteen 3'-fluororibose purine nucleosides 1-15 and eight 3'-fluororibose 2-chloro/2-aminopurine nu...

Eight indole derivatives were studied for their DNA binding ability using fluorescence quenching and molecular docking methods. These indole compounds have structural moieties similar as in few indole alkaloids. Experimental and theoretical studies have suggested that indole derivatives bind in the minor groove of DNA. Thermodynamic profiles of DNA complexes of indole derivatives were obtained ...

Exogenous purines are known to suppress purine synthesis de novo in bacteria (2-4), HeLa and L cells (5), and ascites cells (6) growing in culture. High levels of purines, especially adenine, inhibit accumulation of late purine precursors in the media of bacteria with blocks in the pathway of purine synthesis (2, 3), or of formylglycinamide ribonucleotide in Ehrlich ascites cells grown in the p...

The phase transfer method was applied to perform the nucleophilic substitution of 2,6-dichloropurines by modified arylalkyl alcohol or phenols. Since under these conditions only the 6-halogen is exchanged, this method gives 2-chloro-6-aryloxy- and 2-chloro-6-arylalkoxy-purines. 2-Chloro-6-benzylthiopurine was synthesized by alkylation of 2-chloro-6-thiopurine with benzyl bromide. The stereoisom...

Ultraviolet photoelectron spectroscopy has been employed to study electronic structure and tautomerism in adenine and purine and in various methyl-substituted derivatives of these molecules. The assignment of the photoelectron spectra has been aided by results from HAM^ molecular orbital calculations which are empirically parameterized to provide orbital ionization potentials. The calculations ...

The previous studies in a series of 8-methoxy-1,3-dimethyl-2,6-dioxo-purin-7-yl derivatives revealed their analgesic properties. We extended the study with these compounds in aim to assess their impact on inflammatory process. For this purpose we used: the zymosan-induced peritonitis and the carrageenan-induced edema model. Furthermore, the antioxidant activity of the investigated compounds by ...