Since the purpose of this paper is to record the cultivation of amoebae upon autolyzed tissue without bacterial association, the morphological characteristics, life cycle, means of differentiating species, and pathogenicity of the protozoa have been omitted. These subjects will be considered in later publications. The result of this study proves that some species of amoebae from liver abscesses...

The syntrophically propionate-oxidizing bacterium Syntrophobacter wolinii was grown in binary methanogenic coculture with Methanospirillum hungatei as sole partner, free of contaminating Desulfovibrio vulgaris. Substrate tests revealed that S. wolinii could grow also without any syntrophic partner, either with pyruvate as sole substrate or with propionate plus sulfate. Pyruvate was fermented to...

Some media are described which inhibit bacterial growth, but are favorable to protozoan development. A purification technique, which takes advantage of geotropic responses, was devised and used successfully with 7 species of protozoa, including flagellates and ciliates. The method was also used with a Spirillum. For one flagellate which could not be purified in this manner, a procedure involvin...

COIIMAN, M. D., BLEDSOE, C. S., and LOPUSHINSKY, W. 1989. Pure culture response of ectomycorrhizal fungi to imposed water stress. Can. J. Bot. 67: 29-39. The ability of ectomycorrhizal fungal isolates to tolerate imposed water stress in pure culture was examined in 55 isolates of 18 species. Water potential treatments, adjusted with polyethylene glycol, were applied to Petri dish units. These u...

Bioluminescence methods have been applied to the measurement of the viable and total cell masses of small samples of dental plaque. The total adenine nucleotide content of dental plaque samples and of a pure culture of bacteria was determined and the adenylate energy charge calculated from this. When a pure culture of bacteria was killed with heat, the adenylate energy charge decreased exponent...