The aim of this study was to sequence the genome of the plant growth-promoting Pseudomonas sp. strain CMAA 1215, an osmotolerant bacterium isolated from mangrove soil.

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance have made the expression of stable and active Pseudomonas protein possible in bot...

The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined by using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3-14C]glyphosate revealed that approximately 50 to 59% of the C-3 carbon was oxidized to CO2. Fractionation of stationary-phase cells labeled with [3-14C]glyphosate revealed that from 45 to 4...

region. regulatory oxidative operon, and analysis of the activator of the tcbCDEF chlorocatechol P51 gene tcbR, a LysR-type transcriptional Characterization of the Pseudomonas sp. strain

Background. Bioactive microbial metabolites provide a successful source of novel compounds with pharmaceutical potentials. The bacterium Pseudomonas sp. In5 is a biocontrol strain isolated from a plant disease suppressive soil in Greenland, which produces two antimicrobial nonribosomal peptides (NRPs), nunapeptin and nunamycin. Methods. In this study, we used in vitro antimicrobial and anticanc...

Propachlor (2-chloro-N-isopropylacetanilide) is an acetamide herbicide used in preemergence. In this study, we isolated and characterized a soil bacterium, Acinetobacter strain BEM2, that was able to utilize this herbicide as the sole and limiting carbon source. Identification of the intermediates of propachlor degradation by this strain and characterization of new metabolites in the degradatio...

Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...

Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated J64T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4-37 degrees C, at pH 5.5-10.0 and with 0-10 % NaCl. The strain was found to be oxidase- and catalase-positive. Phylogenetic analyses showed that strain J64T belongs to the genus Pseudomonas, forming a monophyletic group wi...

Pseudomonas putida F1 and Pseudomonas sp. strain JS150 initiate toluene degradation by incorporating molecular oxygen into the aromatic nucleus to form cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene. When toluene-grown cells were incubated with 2- and 3-nitrotoluene, the major products identified were 2- and 3-nitrobenzyl alcohol, respectively. The same cells oxidized 4-nitrotoluene to 2-methyl-...