The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through asymbiotic seed germination technique Mitra medium supplemented with cytokinins (Kn - 4.65 μM, BAP 4.44 μM), and auxin (NAA- 5.37 μM). frequency initiation higher NAA augmented seedlings developed 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication...

A protocol for regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly via indirect protocorm like body (PLBs) formation. The PLBs procured from in vitro growth cultures assessed ½ MS medium alone and combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) α-Naphthalene acetic acid (NAA). calli were induced longitudinally bisected segments PLBs. optimum p...

The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem's Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusA gene expres...

Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order to reveal the lethal an...

Technique for rapid mass propagation of Geodorum densiflorum (Lam)Schltr. has been developed by using thin sections of stems of in vitro regenerated plantlets as explant source. Thin sections of stems (1.0-1.5 mm) when cultured in modified liquid and semisolid Knudson C (KnC) medium produced 1.8 and 1.2 protocorm like bodies (PLBs) per explant respectively. Peptone (2 g l-1) was effective in pr...