Pieris brassicae is one of the most serious pests of cultivated crucifers in the world. The present experiment is intended to determine the proteolytic digestion of fifth instar larvae and the effects of four cruciferous hosts to P. brassicae nutritional physiology. Brassica oleracea var. botrytis, Brassica oleracea var. capitata, Brassica oleracea var. italica and Brassica oleracea var. viridi...

platanus acerifolia, london plane tree, a significant source of airborne allergens, is widely grown in shanghai and other cities in china. in recent decades, little has been known regarding the influence of the allergens on sensitizing the population in the shanghai area. the aims of this study were to purify and characterize the two major allergens and to confirm the immunological activities o...

Illicium verum (IV; dried fruit), Psidium guajava (PG; leaves), and Curcuma longa (CL; rhizome) water extract and/or fish collagen were added into yogurt to determine their effects on acidification the proteolysis of milk up 21 days storage at 4°C. The angiotensin-I converting enzyme (ACE) test was conducted analyze inhibitory activity bioactive peptides produced during proteolytic ACE enzyme. ...

Our interest was stimulated by observing the active proteolytic abilities of a culture of Pseudomonas fluorescens isolated from a frozen chicken pie. The culture liquified gelatin rapidly and attacked casein strongly. In addition, the culture displayed considerable lipolytic and amylolytic activity. The culture was isolated from a tryptone-glucose-meat-extract-agar plate after incubation at 5 C...

The cultivated mycelium of a Cordyceps sinensis (Cs) fungus was sequentially extracted by petroleum ether, ethyl acetate (EtOAc), ethanol and water. The EtOAc extract showed the most potent cytotoxic effect against the proliferation of human premyelocytic leukemia cell HL-60, with an ED50 < or = 25 microg/ml for 2-day treatment. The EtOAc extract induced the characteristic apoptotic symptoms in...

Guinea pig testicles were extracted with acetic acid; the extract was purified by removing material in consecutive precipitations with 30 per cent saturated ammonium-sulfate, trichloracetic acid, and chloroform. The solution so purified, when administered with complete adjuvants, was highly active in inducing impairment of spermatogenesis in guinea pigs. The activity resisted autoclaving at 15 ...

Abstract The viscera and other residues from fish processing are commonly discarded by the fishing industry. These by-products can be a source of digestive enzymes with industrial biotechnological potential. In this study, we aimed at extraction, characterization, application acidic proteases stomach Carangoides bartholomaei (Cuvier, 1833). A crude extract stomachs was obtained submitted to par...

Occurrence of proteolytic enzymes in milk is often associated with technological problems and sensory, rheological and functional defects of final dairy products. Thus, the simple, cost-effective and available laboratory method for evaluation of undesirable proteolysis risk is needed. In our work we have tested cultivation plate methods and chemical methods (formol titration, ammonium reflectom...

A selective and differential growth medium was developed for detection of Clostridium botulinum types A, B, and F. The medium consisted of peptone-glucose-yeast extract agar supplemented with cycloserine, 250 micrograms/ml; sulfamethoxazole, 76 micrograms/ml; and trimethoprim, 4 micrograms/ml as selective inhibitors and various types and levels of botulinal antibodies for type differentiation i...