The kinetics of cell growth and protein production for the recombinant Aspergillus niger strain AB4.l(pgpdAGLAGFP]#II were investigated. An zcnstructured kinetic model was developed to describe the cell grolvth and product formation nmtl~enzatically. The dynamics of glucose consumption and biomass production can be I$-ell-described by the model. The asstcmption that the degradation of GFP by pr...

BACKGROUND As a drug target and an antigenic agent, HIV-1 protease (HIV-1 PR) is at the center of attention for designing anti-AIDS inhibitors and diagnostic tests. In previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in E. coli and invest...

For production of protease by a new strain, Serratia marcescens SB08, optimization of the fermentation medium and environmental conditions, were carried out by applying factorial design and response surface methodology. The results of factorial design showed that pH, agitation, incubation time and yeast extract were the key factors affecting protease production. The optimal cultural conditions ...

Soil samples of Tehran jungle parks were screened for proteolytic Bacilli. Among eighteen protease producers one of the isolates obtained from Lavizan park, in north east of Tehran, was selected for further experimental studies. This isolate was identified as Bacillus sp. strain CR-179 based on partial sequencing of 16S rRNA. Various nutritional and environmental parameters affected protease pr...

The protease myroilysin is the most abundant protease secreted by marine sedimental bacterium Myroides profundi D25. As a novel elastase of the M12 family, myroilysin has high elastin-degrading activity and strong collagen-swelling ability, suggesting its promising biotechnological potential. Because myroilysin cannot be maturely expressed in Escherichia coli, it is important to be able to impr...

Comparative study was carried out on the production of protease using isolate from moraine. The isolates were screened on gelatin plates for gelatin hydrolyses by the different isolates. Among the all tested isolate No. 7 EHN produced the highest activity. while under the fermentation conditions after 3 days the maximum yield of protease was 277 U/ml. The protease production from marine isolate...

This study was conducted to investigate the effects of protease supplementation on behaviour, slaughter performance, meat quality, and immune organ indices broilers. A total 240 one-day-old Arbor Acres broiler chickens were randomly allocated four treatments, with six replicates per treatment 10 broilers replicate. Chickens fed corn-soybean meal basal diets supplemented 0, 4, 8, 12 IU/g p...

Background: Proteolytic enzymes have an important role in variety of physiological and pathological functions. They have been used in therapeutic and pharmaceutical applications. Characterizations of extracellular proteases from various strains of S. marcescens indicate that most strains produce a very similar major metalloprotease. This metalloprotease (serrapeptidase, serrapeptase) is an impo...

Bacillus strains isolated from the salteren pond (Kakinada) were screened and identified for high alkaline protease activity. The isolates which were positive on skim milk agar (1%) were selected as protease producing strains. Of the ten bacterial isolates screened, isolate S-8 was observed as a potential haloalkaline protease producer and it was identified as Bacillus cereus strain S8 (MTCC NO...

The production of protease by isolated aleurone layers of barley in response to gibberellic acid has been examined. The protease arises in the aleurone layer and is mostly released from the aleurone cells. The courses of release of amylase and protease from aleurone layers, the dose responses to gibberellic acid and the effects of inhibitors on the production of both enzymes are parallel. As is...