Background: Cytoplasm and mitochondria are considered as the major origins of sperm ROS production. Sperm is prone to DNA damage by exposure to ROS or due impaired chromatin remodeling or low DNA protamination. Therefore, the aim of this study was to see if there is any association between impaired chromatin packaging and origin of ROS production. Materials and Methods: Cytosolic ROS, mitochond...

BACKGROUND When used to reverse the anticoagulant effect of heparin, protamine sulfate often causes vasodilation that can lead to systemic hypotension. Protamine is rich in the basic amino acid arginine, which is the precursor of endothelial cell synthesis of nitric oxide, and nitric oxide is the active component of endothelium-derived relaxing factor (EDRF). METHODS AND RESULTS To determine ...

This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and a...

comparison with the negative control was scored as positive. Furthermore, in vitro allergy testing using the Phadia CAP system for specific IgE against shrimp, fish-mix, rainbow trout, rCyp p 1 and protamine sulfate was performed. Results: Skin prick tests were positive to shrimp, fish-mix, mackerel, salmon and protamine sulfate. Intradermal testing with protamine showed a wheal diameter of 7 m...

Background: Antipsychotic medication use is a common cause of hyperprolactinemia that shown to have adverse effects on male fertility. Risperidone is a combined serotonin/dopamine receptor antagonist that can cause elevations in prolactine level. Its mechanism of cytotoxic effects on testicular germ cells is not fully understood. In the present study we sought to elucidate the impact of risperi...

Background. When used to reverse the anticoagulant effect of heparin, protamine sulfate often causes vasodilation that can lead to systemic hypotension. Protamine is rich in the basic amino acid arginine, which is the precursor of endothelial cell synthesis of nitric oxide, and nitric oxide is the active component of endothelium-derived relaxing factor (EDRF). Methods and Results. To determine ...

The influence of extracytoplasmic proteases on the resistance of Escherichia coli to the antimicrobial peptide protamine was investigated by testing strains with deletions in the protease genes degP, ptr, and ompT. Only DeltaompT strains were hypersusceptible to protamine. This effect was abolished by plasmids carrying ompT. Both at low and at high Mg2+ concentrations, ompT+ strains cleared pro...

Introduction: Protamine is widely used to reverse heparin. However, protamine administration may cause systemic hypotension, pulmonary hypertension, anaphylactic shock, and cardiovascular collapse. (1) In one recent pediatric study, the only reaction seen was systemic hypotension. This was associated with small heparin dose, large protamine dose, and female gender with an incidence of 1.76%-2.8...