The aim of our study was to consider the efficiency of randomly amplified polymorphic DNA (RAPD) analysis to show the differences between Rhododendron mutants and control plants. The mutant plantlets were selected due to their better In vitro shoot and root regeneration relative to control in both varieties. Six mutant plantlets from Alfred and seven mutant plantlets from Paars variety were sel...

Morphological, anatomical and physiological features of in vitro plantlets are affected by the environmental conditions experienced. The high water content inside in vitro-formed tissues makes difficult to maintain the original characteristics of samples from in vitro plantlets during the preparation procedures for morphological and anatomical studies. In this paper, micropropagated Vitis vinif...

Plant somatic cells have the remarkable ability to regenerate an entire organism. Many species in the genus Kalanchoë, known as "mother of thousands," develop plantlets on the leaf margins. Using key regulators of organogenesis (STM) and embryogenesis (LEC1 and FUS3) processes, we analyzed asexual reproduction in Kalanchoë leaves. Suppression of STM abolished the ability to make plantlets. Here...

As interest in how to increase biomass production through biotechnological means gains traction, focus is turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevated CO2 with cold cathode fluorescent lamps (CCFLs) on the photoautotrophic growth of Oncidesa in vitro has been stu...

An autotrophic culture system was developed for the in vitro mycorrhization of potato plantlets. Roots of micropropagated plantlets were associated to an arbuscular mycorrhizal fungus under in vitro conditions, while shoots developed under open air conditions. Several thousand spores, an extensive extraradical mycelium and an abundant root colonization were obtained. Spores were able to coloniz...

Date palm tissue culture technique aimed to production of huge numbers of plants to farmers in order to increasing dates which have high nutritional value. Date palm plantlets from pre-acclimatization stage in vitro were transferred to acclimatize in the greenhouse cultured in peat moss + perlite 2:1 and kept in the plastic covered inside 100% humidity and 28 C0 till new leave or root was grown...

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted unde...

Zygotic embryos excised from immature green fruits of the rattan palm, Calamus thwaitesii and cultured for 16 weeks under optimum culture conditions in Murashige and Skoog (MS) medium supplemented with 31.67 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 35.23 μM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) produced mixed (compact and friable) calli at 70 and 92 % rates. The semi-friable part of ...

Philodendron bipinnatifidum Schott ex Endl. is a popular ornamental plant that normally propagated by tissue culture methods. However, the growth and acclimatization of micropropagated plants are tarrying processes. Therefore, in present study we examined effect arbuscular mycorrhizal fungi (AMF) Gigaspora albida G. marginata on success establishment, growth, development P. plantlets during pha...

The influences of flax lignin and phenolic compounds obtained from spruce bark on the development of Lycopersicon esculentum plantlets were evaluated. Depending on the applied treatment and the concentrations used, the natural aromatic compounds had stimulatory effects on the germination capacity and the plantlets height and leaf area. The influence of lignin on Lycopersicon esculentum was lowe...