two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40 min, cont...

dog rose (rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. it can be propagated under in vitro conditions. after removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on murashige and skoog medium (ms ...

given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. the present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...

objective(s):in previous investigations, we have shown para-nonylphenol (p-np) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (mscs) in vitro. in this study, we first treat the rats with p-np, then carried out the biochemical and morphological studies on mscs. materials and methods: proliferation property of cells was evaluated with t...

two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40...

introduction : deproteinized bovine bone mineral (bio-oss) is a xenogenic bone substitute, widely used in maxillofacial bone regeneration. the aim of this in vitro study was to investigate its influence on the growth behavior of human osteosarcoma cell line, saos-2 culture, and compare it with the physiologic dose of dexamethasone, an inductive factor for osteoblasts. materials and methods: hum...

background: the appendix is considered as part of the gut-associated lymphoid tissue; however, lymphocyte subsets in this tissue are not fully defined. objective: to investigate and compare the function and phenotype of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed appendix tissues. methods: peripheral blood samples and appendiceal mononuclear cells we...

background: iron is an essential trace element in cell proliferation. several investigations demonstrate that iron deprivation inhibits cell proliferation. however, the impact of iron on telomerase activity of activated lymphocytes remains unexplained to date. objective: in this study, the effect of iron on the proliferation and telomerase activity of lymphocytes stimulated by phytohemagglutini...

background and objective: the association of ebv with hodgkin lymphoma (hl) has been intensely investigated over the last few years. ebv is also associated with several other malignancies. on the other hand, ki67 molecule serves as a widely accepted proliferation marker. several studies were previously performed about the expression of ki67 in hd. this study tried to detect the correlation betw...

in vitro expansion of mesenchymal stem cell (mscs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. on the other hand, some studies have indicated that bio (6-bromoindirubin-3-oxime) possesses mitogenic effects on cell culture. the objective of the present study was to examine the effect of bio on in vitro expansion and chondrogenic di...