BACKGROUND Mycoplasma genitalium causes urogenital diseases in men and women and is presumed to be sexually transmitted. We wanted to investigate whether spermatozoa could serve as vectors for M.genitalium in order to cause upper genital diseases in women. METHODS By use of Nomarski light microscopy and transmission X-ray microscopy, the attachment of M.genitalium to spermatozoa was studied. ...

The effect of in-vitro culture on the motility and morphology of fresh and frozen-thawed human testicular spermatozoa obtained from obstructive azoospermic patients and on the motility of testicular spermatozoa obtained from non-obstructive azoospermic patients was evaluated. The outcome of intracytoplasmic sperm injection (ICSI) with fresh and frozen-thawed human testicular spermatozoa was stu...

Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected o...

To identify Kraft liquor effects on spermatogenesis and sperm motility of Caspian kutums by using in vitro culture, the fish testis was incubated with 5, 10 and 15 % (V/V) of liquor for 3 and 6 days. The percentage of motile spermatozoa and its total duration were also measured. Adverse effects on testicular cells were more apparent with the increase of liquor concentrations. The mean size of s...

this study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. ejaculates were collected from four holstein bulls, twice a week during four weeks. semen samples diluted with bioxcell extender containing zero (control), 10-6, 10-5, 10-4, 10-3 and 10-2 m of zinc nano-complex. the samples we...

Progressively motile spermatozoa were present in 42/47 (89%) of post-vasectomy specimens, and in 16 of 24 samples (66%) demonstrable fertilizing potential could be detected in the zona-free hamster egg assay. The mean +/- s.e.m. post-vasectomy penetration rate of 20.6 +/- 6.0% was, however, significantly lower (P less than 0.001) than the pre-vasectomy value of 49.7 +/- 6.4%. After vasectomy th...

This experiment was designed to study the effect cooling rate in Hariana bull spermatozoa during cryopreservation. For this purpose, ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen sample which possesses more than 70% progressive motility and above 500 million/ml spermatozoa concentration was subsequently subjected to processing for liqu...

To identify Kraft liquor effects on spermatogenesis and sperm motility of Caspian kutums by using in vitro culture, the fish testis was incubated with 5, 10 and 15 % (V/V) of liquor for 3 and 6 days. The percentage of motile spermatozoa and its total duration were also measured. Adverse effects on testicular cells were more apparent with the increase of liquor concentrations. The mean size of s...

Hamster spermatozoa were isolated from the caput, corpus and cauda epididymidis. They were observed in culture medium at 37 degrees C with a phase-contrast microscope and their motility recorded cinematographically. About 20 p. 100 of the caput epididymidis spermatozoa were motile and moved in a confined space with no forward progression. 30 p. 100 of the corpus epididymidis spermatozoa were mo...

The effect of porcine reproductive and respiratory syndrome virus (PRRSV) on semen quality was examined in a group of 11 spontaneously infected boars in a commercial boar stud. Semen samples were collected 4 weeks prior to 4 weeks post-infection (wpi). Infection with PRRSV of the European genotype subtype 1 (EU-1) was verified by specific quantitative real-time polymerase chain reaction (RT-PCR...