The prM protein of Japanese encephalitis virus (JEV) contains a single potential N-linked glycosylation site, N(15)-X(16)-T(17), which is highly conserved among JEV strains and closely related flaviviruses. To investigate the role of this site in JEV replication and pathogenesis, we manipulated the RNA genome by using infectious JEV cDNA to generate three prM mutants (N15A, T17A, and N15A/T17A)...

UNLABELLED The proposed antibody-dependent enhancement (ADE) mechanism for severe dengue virus (DENV) disease suggests that non-neutralizing serotype cross-reactive antibodies generated during a primary infection facilitate entry into Fc receptor bearing cells during secondary infection, resulting in enhanced viral replication and severe disease. One group of cross-reactive antibodies that cont...

The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review d...

This paper applies Probabilistic Relational Models (PRMs) to the Collaborative Filtering task, focussing on the EachMovie data set. We first learn a standard PRM, and show that its performance is competitive with the best known techniques. We then define a hierarchical PRM, which extends standard PRMs by dynamically refining classes into hierarchies, which improves the expressiveness as well as...

background: paradoxical reactions to benzodiazepines including restlessness, anxiety and sometimes violent behavior sometimes occur. most of the known predicting factors of disinhibitory reactions such as age, gender, genetic or the psychological backgroundare not modifi able. th is study was conducted to evaluate the eff ect of rate of midazolam administration, as a controllable factor, on the...

Probabilistic Roadmap (PRM) planners have been used to generate paths for articulated robots for several years. By using random sampling techniques, PRM based planners are able to plot paths for robots with many degrees of freedom without needing to explore large parts of the search space that traditional planners would have to examine to create efficient paths. This has enabled them to be used...

We present, implement, and analyze a spectrum of closely-related planners, designed to gain insight into the relationship between classical grid search and probabilistic roadmaps (PRMs). Building on quasi-Monte Carlo sampling literature, we have developed deterministic variants of the PRM that use low-discrepancy and low-dispersion samples, including lattices. Classical grid search is extended ...

The DenV pre-membrane protein (prM) is a crucial chaperone for the viral envelope protein, preventing premature fusion with vesicles during viral export. prM molecules in immature particles are cleaved by host proteases, leading to mature fusogenic virions. Blockade of prM cleavage would restrict fusion and represents a novel druggable opportunity against DenV. We have thus established a cell-b...

Physical and Rehabilitation Medicine (PRM) is an independent medical specialty focusing on the improvement of functioning. A shared understanding of concepts is of vital importance for integrated action in this field. The aim of the present paper is to provide a conceptual model of PRM, to give background on its development and adoptions, and to explain the choice of terms, phrases, and concept...