BACKGROUND Autotransplantation of cryopreserved ovarian cortex can be associated with a risk of cancer cell reseeding. This issue could be eliminated by grafting isolated preantral follicles. Collagenase NB6 is an enzyme produced under good manufacturing practices (GMP) in compliance with requirements for tissue engineering and transplantation in humans and thus can be used to isolate preantral...

A search for genes expressed more highly in mouse cumulus cells than mural granulosa cells by subtraction hybridization yielded Slc38a3. SLC38A3 is a sodium-coupled neutral amino acid transporter having substrate preference for l-glutamate, l-histidine, and l-alanine. Detectable levels of Slc38a3 mRNA were found by in situ hybridization in granulosa cells of large preantral follicles, but level...

background: in vitro maturation (ivm) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). reliable ivm is an intellectual, scientific and clinical challenge with a number of potential applications. objective: the effect of hcg was evaluated on the timing and regulation of in vitro ovulation for the syrian mice oocytes in the pre...

Vitrification of preantral follicles is a promising technique to preserve female fertility. The aim the present study was evaluate effect supplementation α-tocopherol in vitrification solution on viability, lipid peroxidation and mRNA expression superoxide dismutases (SOD1 SOD2) vitrified cultured ovine at day-6 day-12. Preantral (200-300 µm) were isolated from ovaries by mechanical method dist...

Developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. To accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and FSH concentrations. Six-week-old preantral follicles (95 ± 5 µm) were cultured...

Background: Leukemia inhibitory factor (LIF) is a pleiotropic cytokine of interleukin-6 family with a remarkable range of biological actions such as proliferative effects on the granulosa and theca cells. The aim of this study was to evaluate the effects of LIF on the growth and maturation of mouse fresh and vitrified preantral follicles, an in vitro model was developed. Methods: The ovaries of...

Background: α-Lipoic acid (LA) is a cofactor in mitochondrial multienzyme complexes and is well-known for its antioxidant properties in both of in vivo and in vitro condition. Materials and Methods: Preantral follicles were isolated from 2 weeks old NMRI mice ovaries. LA at concentrations of 0 and 100 μM was supplemented to culture media (α-MEM medium supplemented with 5% FBS, 100 mlU/ml FSH, 1...

OBJECTIVE Improvements in cancer treatment have allowed more young women to survive. However, many cancer patients suffer from ovarian failure. Cryopreservation is one of the solutions for fertility restoration in these patients. The cryopreservation of isolated follicles is a more attractive approach in the long term. Many endocrine and paracrine factors can stimulate the granulosa cells of pr...

Follicle and oocyte growth is under the control of systemic gonadotropins and various local intraovarian factors. The present study was undertaken to examine the efficacy of peptide(s) isolated from ovine follicular fluid on preantral and antral follicles, oocytes, cumulus and granulosa cells, and oviductal epithelial cell growth in vitro in a heterologous species (Bubalus bubalis). Follicular ...