The use of syn-tasiRNAs has been proposed as an RNA interference technique alternative to those previously described: hairpin based, virus induced gene silencing or artificial miRNAs. In this study we engineered the TAS1c locus to impair Plum pox virus (PPV) infection by replacing the five native siRNAs with two 210-bp fragments from the CP and the 3´NCR regions of the PPV genome. Deep sequenci...

Plum pox virus (PPV) populations from peaches are able to adapt consistently to herbaceous hosts, characterized by a reduction in time to symptom development, increases in inoculation efficiency and increased titres. PPV adaptation was studied by using pea (Pisum sativum) as an alternative host. Two isolates of PPV from peaches were inoculated and passaged in peas ten times using either aphid o...

The potential of using compact discs as high throughput screening platforms for DNA microarraying is discussed and applied to discriminate genetic variations of Plum pox virus.

M. PLUM Mathematisches Institut I, Universität Karlsruhe, Kaiserstrasse 12, D-76128 Karlsruhe, Germany [Received on 10 December 2002; revised on 12 January 2004] It is well known that routine methods of computing the spectrum of a self-adjoint operator often lead to spurious eigenvalues in a gap between two parts of the essential spectrum. We provide a geometrical explanation for a numerical me...

The reaction of the transgenic plum pox virus (PPV) resistant C-S plum was studied under conditions of co-infection with Prunus necrotic ringspot virus (PNRSV) and PPV as may he encountered in the field. C-S and controls were inoculated with PPV and PNRSV following several co-infection schemes. The results of this preliminary study showed that, as expected, the transgenic clones, including C-S ...

Plum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named 'Jojo', develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infe...

Plum pox virus (PPV), a destructive and economically devastating pathogen of Prunus species, was recently discovered in Pennsylvania and Canada. Current containment efforts involve eradication of infected trees based on ELISA surveys, which are laborious and less sensitive than PCR-based techniques. A real-time, fluorescent, reverse transcription-polymerase chain reaction (RT-PCR) assay was dev...

A TaqMan real-time RT-PCR was developed to detect and quantify RNA-targets from the non-circulative, non-persistently transmitted Plum pox virus (PPV) in individual fresh or aphids captured previously and squashed on paper. Reliable quantitation ranged from 40 up to 4 x 10(8) copies of control transcripts. This technique was applied successfully to plant material and to individual PPV vector (M...

ABSTRACT Plum pox potyvirus (PPV) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. Monoclonal antibodies specific for the two major groups of isolates, represented by the D and M serotypes of the virus, have been obtained. Polymerase chain reaction (PCR)-based assays allowing the direct detection and differentiation of PPV isolates h...

The aim of the study was to find out the host specificity of pigeon pox virus-1 (PPV-1) and fowl pox virus-2 (FPV-2) following cross infection with field strains from Bangladesh. The cross infection was investigated in five groups (n=5) of pigeons and seven groups of chickens (n=5) four weeks of age. Irrespective of route of inoculation each bird received either 0.1 mL of PPV-1 or FPV-2 having ...