We show here that the pvr2 locus in pepper, conferring recessive resistance against strains of potato virus Y (PVY), corresponds to a eukaryotic initiation factor 4E (eIF4E) gene. RFLP analysis on the PVY-susceptible and resistant pepper cultivars, using an eIF4E cDNA from tobacco as probe, revealed perfect map co-segregation between a polymorphism in the eIF4E gene and the pvr2 alleles, pvr2(1...

Viral diseases are major constraints to tomato, cucumber and mung bean production in most areas where these crops grown. To identify the viruses on Tajikistan, a field survey was conducted 2019. Samples of cucumber, tomato with virus-like symptoms were collected present diagnosed by RT-PCR PCR. Across all samples, very high proportion samples infected from genera Cucumovirus Potyvirus. Cucumber...

UNLABELLED The structural pattern of infectivity matrices, which contains infection data resulting from inoculations of a set of hosts by a set of parasites, is a key parameter for our understanding of biological interactions and their evolution. This pattern determines the evolution of parasite pathogenicity and host resistance, the spatiotemporal distribution of host and parasite genotypes, a...

A yeast homologous recombination system was used to generate mutants and chimeras in the genome of Potato leafroll virus (PLRV). A yeast-bacteria shuttle vector was developed that allows mutants and chimeras generated in yeast to be transformed into Escherichia coli for confirmation of the mutations and transformed into Agrobacterium tumefaciens to facilitate agroinfection of plants by the muta...

Potato Virus Y (PVY) is a member of the Potyviridae family – the largest plant virus family. PVY infects Solanaceae – agriculturally important species such as tobacco, potato, pepper, tomato This pathogen causes decrease in yield and quality. PVY isolates have been divided into three main strains: PVYN, PVYO and PVYC, according to the symptoms induced on tobacco and potato. PVYN isolates can be...

Potato production is directly infl uenced by various biological factors among which the most important viruses take the place. Th ere are more than 20 known viruses that aff ect potato causing malformation in growth and yield reduction. In order to preserve the native potato cultivar ‘Poluranka’, which is historically dominant cultivar in the mountainous region of Herzegovina (western part of B...

Potato leafroll virus (PLRV) is transmitted by aphids in a persistent manner. Although virus circulation within the aphid leading to transmission has been well characterized, the mechanisms involved in virus recognition at aphid membranes are still poorly understood. One isolate in our collection (PLRV-14.2) has been shown to be non- or only poorly transmitted by some clones of aphids belonging...

The performance of seed tubers harvested from potato plants grown from sprout cutting, stem cutting and conventional seed tubers against PVY and PLRV were investigated. Sprout cutting, stem cutting and conventional seed tubers of variety Diamant were planted and second and third generation seed tubers were harvested. The least incidence of PVY and PLRV was recorded when seed tubers from sprout ...