Potato viruses cause enormous economic loss in agriculture production. Potatoes can be infected by a number of different viruses that affect negatively the harvest and the tuber quality. Direct and effective drugs against plant virus diseases are still not available and control is only applied as agricultural measures and pesticides against virus vectors. Potato virus Y (PVY) is transmitted by ...

Proteinase inhibitors are ubiquitous proteins that block the active center or interact allosterically with proteinases and are involved in plant physiological processes and defense responses to biotic and abiotic stresses. The CmSPI gene identified from Cucumis metuliferus encodes a serine type PI (8 kDa) that belongs to potato I type family. To evaluate the effect of silencing CmSPI gene on Pa...

The cucumber mosaic virus (CMV) 2b viral suppressor of RNA silencing (VSR) inhibits host responses to jasmonic acid (JA), a chemical signal regulating resistance to insects. Previous experiments with a CMV subgroup IA strain and its 2b gene deletion mutant suggested that VSRs might neutralize aphid (Myzus persicae) resistance by inhibiting JA-regulated gene expression. To further investigate th...

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

Background: Potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. Potyviruses can be transmitted through plant sap, seeds and many aphid species. Due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. Objectives: This study compared the efficiencies of two couples of primers in the...

EPPER vein-banding virus causes a troublesome disease of cultivated peppers I? (Capsicum spp.) in Puerto Rico (ADSUAR 1940) and in Trinidad. A rather similar disease occurs in Hawaii ( KIKUTA 1947). Serological investigations (PEREZ and ADSUAR 1955) indicate that the virus responsible for the West Indian disease is a strain of potato virus Y. In Trinidad, DALE (1954) found that three distinct s...

Potato virus Y (PVY) coat protein gene has been cloned from an Indian isolate of PVY. The coat protein gene from this isolate showed 93.2% nucleic acid sequence homology and more than 94% amino acid sequence homology compared to an American isolate. The coat protein gene has been sub-cloned into plant expression vector pBINPLUS and tobacco leaf discs were transformed using Agrobacterium tumefac...

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing (32)P labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of act...