Ascochyta blight (caused by rabiei) is an important disease of chickpea. Mating type distribution, genetic diversity and population structure A. rabiei isolates from western Iran, using specific matting primers, ISSR SSR molecular markers. Two mating types were identified, with the 57% belonging to MAT1-1. Ten markers produced 78 polymorphic bands average polymorphism information content (PIC) ...

This study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations universal rice primers (URPs). A total number 37 URP marker pairs were tested twenty those provided amplicons in genome. The amplified markers a 83 bands 62 them determined as polymorphic scattered whole average polymorphism rate was calculated 73.5% while informat...

The aim of the present study was to evaluate genotypic distribution calpastatin (CAST) and insulin-like growth factor 1 (IGF1) gene polymorphisms in Tsigai sheep breed. Genomic DNA isolated from blood samples by phenol-chloroform method. A total 56 were genotyped PCR-RFLP Allele genotype frequencies calculated standard procedures. To population-genetic properties, Hardy–Weinberg Equilibrium (HW...

Polymorphism of the second exon of the caprine leukocyte antigen-DRB3 gene (CLA-DRB3*02) was investigated in this study. The 285 bp PCR product of 258 individuals from 10 domestic goat breeds in Southwest China was digested with restriction endonucleases PstI and HaeIII and then genotyped. Three alleles and 4 restriction digestion profiles were distinguished by digestion of the PCR fragment by ...

Parsley (Petroselinum crispum Mill.) is a vegetable that has many benefits for human health, and its cultivation increasing. In order to carry out breeding studies in parsley, genotypes should be characterized terms of some characteristics. this study, phytochemical properties 18 different parsley were revealed, their genetic diversity was determined with the iPBS (Inter Primary Binding Site) m...

The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% p...

Genotyping for five short tandem repeat (STR) loci HUMvWA, HUMFES, HUMTPO, HUMTH01 and D3S1359 was done in 220 unrelated individuals from the population of Isfahan province of IR Iran. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The data demonstrated that the STR markers were all found informative i...

Iron is one of the most important nutrients in the human diet. According to the high consumption of staple foods such as wheat, the deficiency of iron in these crops would lead to nutritional disorders and related complications. To identify microsatellite markers associated with wheat grain iron content,38Iranian prevalent wheat genotypes were assessed using 30 pairs of genomic and EST microsat...

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F(2) progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers produced 261 reproduc...