We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optimal temperature and pH of the protein were 72 °C and 5.2, respectively. CbPelA demonstrated high th...

Alkyl esters of pectin and pectic acid have been prepared via three different synthetic routes. Enhancements of intrinsic viscosity, binding to bile acids and to isolated soy protein were shown for these novel pectin derivatives. The development of new and improved pectin-containing products may be enhanced by the ease of preparation and improved functionality of such compounds.

The water-insoluble fraction, obtained after successive treatment of an insoluble fraction of a buffer-homogenate of Zea mays L. hybrid B73 x Mo 17 shoots with 3 molar LiCl and hot water, was treated with alpha-amylase to remove starch. This fraction has been subjected to sugar composition analysis and the major glycosidic linkages were determined. The results suggest that the water-insoluble f...

Pectinases can be used to remove impurities and hydrophobic material from cotton fibers by the degradation of pectic substances. The biochemical characterization evaluation process parameters that influence enzyme’s activity are mandatory optimize industrial application. In present study, a factorial experimental design was conducted evaluate commercial pectate lyase at different reaction condi...

In 1960 Albersheim, Neukom, and Deuel (1) reported on an enzyme present in a commercial pectic enzyme preparation (Pectin01 RlO, Rohm and Haas Company, Philadelphia) that degraded the a, 1 + 4-glycokidic bonds in pectin by a trans elimination of the proton on the 5th carbon atom of an anhydromethyl galacturonate unit with the oxygen of the adjacent glycosidic bond. Cleavage of the bonds in pect...

The contents of the lower alimentary tract from rats fed a semisynthetic, pectin-supplemented diet showed increased nitrate reductase activity and an increase in the amount of luminal contents in the intestine and cecum. Nitrate reductase activity was associated with the insoluble fraction of the gut contents which was sedimented by centrifugation (5,100 X g,20 min) and was abolished after trea...