background: opticin, as a member of small leucine rich proteoglycans (slrps), mainly expressed in human eyes. it is also expressed in human brain, ligament, liver and skin, but at lower levels than the eyes. in the present study, expression of opticin was studied in a variety of human cancer cell lines. materials and methods: in this basic study, human cancer cell lines including, a-172 (brain ...

background: zoonotic cutaneous leishmaniasis (zcl) is an increasing public health problem in some endemic re­gions. horseradish peroxidase (hrp) conjugated rabbit anti- rhombomys opimus ( r. opimus ) ig is needed for im­munoblotting and elisa tests used to explore the immune response of the rodents against the sand fly saliva. in this study, the production of hrp conjugated rabbit anti- r. opim...

Abstract: The aim of the present study was to produce a polyclonal antibody against bovine serum albumin (BSA) conjugated with artemisinin. To gain an immunogenic character of artemisinin, a carboxyl group was added to it using mixed anhydride method. Then, the reactive compound of artemisinin was conjugated with BSA. The BSA+artemisinin were injected to white female New Zealand rabbits for two...

Virulence of the opportunistic yeast, Candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. Cell wall mannoproteins are found to be the main cause of adherence of C. albieans to epithel ial cells in the first step of an infection process. In the present study, cell wall mannoproteins of intact yeast wer...

r-phycoerythrin (r-pe), a fluorescent protein from phycobiliprotein family, is isolated from red algae. conjugation of antibodies to r-pe facilitates multiple fluorescent staining methods. in the present study polyclonal antibodies and polyclonal f(ab')2 fragment antibodies were conjugated to r-pe by two different methods. the efficiency of the methods was evaluated using immunocytochemistry (i...

Polyclonal antibodies against kappa light chain are used to diagnose diseases producing free light chain. The kappa and lambda light chains are products of immunoglobulin synthesis and released into the circulation in minor amounts such as serum, cerebrospinal fluid, urine and synovial fluid in normal condition. The purpose of this study was the production and purification of polyclonal immunog...

Persistent polyclonal B-cell lymphocytosis (PPBL) is a rare and recently described entity. PPBL is diagnosed predominantly but not exclusively in women, usually smokers and is characterized by a moderate, chronic and absolute lymphocytosis (> 4 x 109/L). Peripheral blood examination show in all cases atypical binucleated lymphocytes. A polyclonal serum IgM is also associated and HLA-DR7 express...