Background and Objectives: Making stacking gels for polyacrylamide gels in the laboratory by conventional methods is laborious and time consuming. Considering the role of temperature in polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing the temperature would cause an increase in electrical resistance and viscosity. Ultimately, a downward temperat...

introduction the purpose of the current study was to determine optical sensitivity of n-isopropyl acrylamide nipam and polyacrylamide gelatin and tetrakis hydroxymethyl phosphoniun chloride (pagat) polymer gels for different wavelength of visible light spectrum applied in optical computed tomography method. materials and methods nipam and pagat polymer gels with conventional formulations used f...

BACKGROUND Prenatal diagnosis for beta thalassaemia has proved to be very effective in preventing the birth of an affected child and hence in controlling the disease. The success of prenatal diagnosis depends on the delineation of the underlying mutations in the population at risk. Each population carries a limited number of frequent defects (89-91%) and a variable number of rare alleles (4-5%)...

This article gives an overview of uncommon replaceable matrices (gels) for capillary gel electrophoresis. This electrophoretic technique is useful mainly for the separation and analysis of biopolymers-nucleic acids and their fragments, and proteins/peptides. Commonly used gels are not reviewed. Those mentioned and discussed here are gels containing saccharides, newly developed acrylamide-based ...

Currently, there is an interest in novel drug delivery systems, diagnostic capabilities and improving separation of biomacromolecules such as DNA and proteins. One possible approach is to add charged nanoparticles to the polyacrylamide gel electrophoresis system to observe the difference in, for example, protein separation efficiency. Another approach included creating templated pores by polyme...

(1983) Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods in Enzymology 91: 227. Laas T (1989) Electrophoresis in gels. In: Janson J-C and Ryden L (eds) Protein PuriTcation } Principles, High Resolution Methods and Applications. New York, Weinheim and Cambridge: VCH Publishers. Laemmli UK (1970) Cleavage of structural proteins during...