Damage to peripheral nerves often cannot be repaired by the juxtaposition of the severed nerve ends. Surgeons have typically used autologous nerve grafts, which have several drawbacks including the need for multiple surgical procedures and loss of function at the donor site. As an alternative, the use of nerve guidance channels to bridge the gap between severed nerve ends is being explored. In ...

In article number 2001448 by Morgan R. Alexander, Chris Denning, and co-workers, a novel substrate for xeno-free human pluripotent stem cell (hPSC) culture is discovered using highthroughput multigenerational polymer microarray platform approach used to rapidly test over 600 polymers. This nanoscale phase separated blend of poly(tricyclodecane-dimethanol diacrylate) poly(butyl acrylate) (2:1 v/...

We report on the use of polyelectrolyte multilayer (PEM) coatings as a non-biological surface preparation to facilitate uniform cell attachment and growth on patterned thin-film gold (Au) electrodes on glass for impedance-based measurements. Extracellular matrix (ECM) proteins are commonly utilized as cell adhesion promoters for electrodes; however, they exhibit degradation over time, thereby i...

Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. ...

Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is an increasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion, dynamic morphology change, and differentiation. In this study, without adding any other bioactive factors in the serum-containing medium, dynamic behaviors of mouse EBs ...

4.1 Molecular biology methods 28 4.1.1 Construction of expression plasmids 28 4.2 Cell biology methods 29 4.2.1 Cell culture and transfection 29 4.2.2 ImmunoFISH 30 4.2.3 Microscopy, image analysis and statistical evaluation 31 4.3 Biochemical methods 32 4.3.1 In vivo protein interaction assays 32 4.3.2 In vitro protein interaction assays 33 4.3.3 Western blot analysis 34 4.3.4 In vitro poly(AD...

A mixed culture of Pseudomonas fluorescens BS2 and Pusillimonas noertemannii BS8 degraded poly-γ-d-glutamic acid; when the 2 strains were cultured separately, no hydrolytic activity was apparent. Here we report the draft genome sequences of both soil isolates.