Poleroviruses are restricted to vascular phloem tissues from which they are transmitted by their aphid vectors and are not transmissible mechanically. Phloem limitation has been attributed to the absence of virus proteins either facilitating movement or counteracting plant defense. The polerovirus capsid is composed of two forms of coat protein, the major P3 protein and the minor P3/P5 protein,...

A fluorescent viral clone of the polerovirus Turnip yellows virus (TuYV) was engineered by introducing the Enhanced Green Fluorescent Protein (EGFP) sequence into the non-structural domain sequence of the readthrough protein, a minor capsid protein. The resulting recombinant virus, referred to as TuYV-RTGFP, was infectious in several plant species when delivered by agroinoculation and invaded e...

Viral synergism is caused by co-infection of two unrelated viruses, leading to more severe symptoms or increased titres of one or both viruses. Synergistic infection of phloem-restricted poleroviruses and umbraviruses has destructive effects on crop plants. The mechanism underlying this synergy remains elusive. In our study, synergism was observed in co-infections of a polerovirus Brassica yell...

With the increasing availability of aphid genomic data, it is necessary to develop robust functional validation methods to evaluate the role of specific aphid genes. This work represents the first study in which five different techniques, all based on RNA interference and on oral acquisition of double-stranded RNA (dsRNA), were developed to silence two genes, ALY and Eph, potentially involved i...

Abstract Pepper vein yellows viruses (PeVYV) are phloem-restricted in the genus Polerovirus , family Luteoviridae . Typical viral symptoms of PeVYV including interveinal yellowing leaves and upward leaf curling were observed pod pepper plants ( Capsicum frutescens ) growing Wenshan city, Yunnan province, China. The complete genome sequence a virus from sample these was determined by next-genera...

Viruses in the genus Polerovirus infect a wide range of crop plants and cause severe economic losses. BrYV belongs to is transmitted by Myzus persicae. However, changes transcriptome proteome profiles M. persicae during viral infection are unclear. Here, RNA-Seq TMT-based quantitative proteomic analysis were performed compare differences between viruliferous nonviruliferous aphids. In total, 12...

The vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects the transmission process being mediated key protein–protein interactions. Still, very few vector proteins interacting have been identified and functionally characterized. Potato leafroll virus (PLRV) is most efficiently Myzus persicae, green peach aphid, in a circulative, non-propagative manner. Usin...