To measure apolipoprotein A-I, we use an RIA kit (Ventrex Laboratories, Inc., Portland, ME) for which serum is recommended, and avoidance of repeated freeze-thaw cycles. With the reported association of alterations in apolipoprotein A-I concentrations in plasma with both heart (1) and liver disease (2), those interested in retrospectively measuring apolipoprotein A-I with this kit have been lim...

OBJECTIVE The mechanisms by which apolipoprotein E (apoE) can promote the regression of atherosclerosis are not well understood. This study examined whether apoE can promote atherosclerosis regression independently of lowering plasma cholesterol levels. METHODS AND RESULTS We studied hypomorphic apoE mice (Apoe(h/h)), which express an apoE4-like form of mouse apoE at approximately 2% to 5% of...

A truncated apolipoprotein (apo) A-I with a molecular weight (M(r)) of 26 kDa was first isolated from the plasma high density lipoproteins of an atypical Japanese eel (Anguilla japonica). Interestingly, this eel contained a very small amount of intact apoA-I (M(r)28 kDa) in the plasma, although serine protease inhibitors were present throughout the plasma preparation. The N-terminal sequence of...

was precipitated (Kane et al., 1975) with 1,1,3,3-tetramethylurea, extracted with chloroform/methanol (1 : 1, v/v), washed with ether, and dried under N2. Amino acid analysis of this protein corresponded to that of apolipoprotein B, as did its electrophoretic mobility on urea/polyacrylamide-gel electrophoresis. Its specific radioactivity was obtained by radioactivity counting and protein determ...

Methimazole-treated hypothyroid rats were injected intravenously with triacylglycerol/cholesteryl oleate/cholesterol/phospholipid emulsions designed to model the composition of chylomicrons. Compared with controls, hypothyroidism decreased the clearance rates of emulsion cholesteryl oleate. Clearance of emulsion triolein was affected much less and could be accounted for by residual triolein in ...

lipoprotein (a) [lp (a) is formed by assembly of ldl-particles and the carbohydrate rich protein, apolipoprotein (a) [apo (a)]. elevated plasma lp (a) levels are an independent predictor of the development of premature coronary artery disease (cad), but is not clear whether the apo (a) isoform plays on additional and independent role or not. to investigate the possible effect of apo (a) isoform...

OBJECTIVE The apolipoprotein AI-CIII-AIV cluster has been associated with the response to a urate-lowering diet, and polymorphisms in the apolipoprotein CIII gene have been associated with hyperuricemia and hypertriglyceridemia. We assessed the influence of polymorphisms in the apolipoprotein AI-CIII-AIV cluster on the response to a urate-lowering diet in patients with hyperuricemia. METHODS ...

Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol...