Mitigation of ethylene promoted leaf senescence by lysophosphatidylethanolamine (LPE) was studied. Micropropagated ‘Russet Burbank’ potato (Solanum tuberosum L.,) plantlets were grown on MS media in sterile culture tubes. After 2 weeks of growth, tubes were sealed and ethylene gas was applied to obtain 5 nL·L fi nal concentration in the culture tubes. Observations and measurements were taken tw...

the purpose of this study was to examine the effectiveness of nanosilver colloid as a new antimicrobial agent against erwinia in tissue culture of sugar beet for the recovery of the contaminated doubled haploid plantlets. the nanosilver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid ms medium. in a factorial experim...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...

The growth of strawberry plantlets in the rooting stage on culture medium supplemented with silver nanoparticles (AgNPs) and ethylene gas accumulation plantlet bottles were investigated. In addition, different systems first used to produce large-scale Strawberry plantlets. results showed that shoots (3 cm) cultured MS 0.02 mg/L NAA, 1 g/L activated charcoal, 30 sucrose, 8 agar 0.5 AgNPs about 4...

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with ...

Continuous production of healthy plantlets of Sapindus trifoliatus L. was achieved via somatic embryos from long term cultures of an embryogenic mass (EM). A highly embryogenic culture of S. trifoliatus L. was obtained by recurrent embryogenesis from somatic embryos cultured on Murashige and Skoog's (MS) medium supplemented with kinetin (2.3 microM) and benzyladenine (8.8 microM). The cultures ...

Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined...