In virology, cell culture usually refers to the in vitro growth and manipulation of cells from a tissue obtained from a multicellular organism. The term “cell culture” is often used interchangeably with “tissue culture”. Cell culture remains integral with virology, as viruses are obligate intracellular parasites that require replication within a living cell to produce copies of themselves (i.e....

Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Role of humic acids (HA) in plant tissue culture as plant growth regulators has been reviewed. Humic acid is a principal component of humic substances, which are the most important organic constituents of the soil (humus). Humus is defined as the organic matter in soil, a mixture of partially and totally humified substances. Leonardite is the best source for humic acid and it is extracted by us...

Traditional plant breeding is often limited by the genetic diversity within a species. The use of biotechnology allows introducing into a plant, specific traits that come from the same or another plant species. In this paper, we focus on tissue culture of sunflower (Helianthus annuus L., Asteraceae), particularly related to rooting transgenic sunflower plantlets. Sunflower is notoriously recalc...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...