In the preceding paper (1) are recorded the facts so far obtained in a study of the proteolytic enzymes of pneumococcus. It has been shown that bile solutions of pneumococci, extracts obtained by disintegration of the organisms in phosphate mixtures, and sterile filtrates of autolyzing broth cultures possess the power to hydrolyze peptones and to a less extent certain intact proteins. In the pr...

Fluorescence is of diagnostic value for differentiating among species of aerobic pseudomonads (R. Y. Stanier, N. J. Palleroni, and M. Doudoroff, J. Gen. Microbiol. 43:159, 1966). The standard medium for detecting fluorescence is Medium B (E. 0. King, M. K. Ward, and D. E. Raney, J. Lab. Clin. Med. 44:301, 1954), which supports amino acids (J. De Ley, Ann. Rev. Microbiol. 18:17, 1964), and pepto...

serum. The maximum cell concentration was a function of the frequency of medium renewal, the time of incubation, and the amount of final cellular material present. Maximum cell density could be increased approximately twofold by the addition of various peptones to the complete medium, although these materials did not affect the minimum generation time. Fortification of the medium with glucose, ...

background: transient gene expression (tge) gained popularity over the last decade as a rapid method for the production of milligram to gram quantities of recombinant proteins for preclinical studies in biophama industry. thereby, the optimization of the tge technique for chinese hamster ovary (cho) as the dominant host for the production of biotherapeutics is of great interest to reach the val...

The strong acids that are required for complete hydrolysis of proteins destroy some amino acids, such as tryptophan, asparagine, and glutamine. Other amino acids are destroyed to a lesser extent or in certain cases are released incompletely from peptide linkage. It is evident that these difficulties, as well as others, could be overcome by employing proteolytic enzymes as the sole agents for hy...

BACKGROUND Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production. MATERIALS AND METHODS Thermophilic amylase prod...